circ-E2F3促进宫颈癌细胞增殖的机制研究  

Mechanism of circ-E2F3 promoting the proliferation of cervical cancer cells

作　　者：缪华珍 刘敏[2] MIAO Huazhen;LIU Min(Jiangyin City Maternal and Child Health Hospital,Wuxi,214400,Jiangsu,China;Nanjing Second Hospital,Nanjing,210003,Jiangsu,China)

机构地区：[1]江阴市妇幼保健院,江苏无锡214400 [2]南京市第二医院,江苏南京210003

出　　处：《肿瘤药学》2023年第4期443-448,共6页Anti-Tumor Pharmacy

摘　　要：目的研究circ-E2F3通过抑制miR-296-5p影响宫颈癌进展的作用机制。方法采用qRT-PCR检测宫颈癌组织和癌旁组织中circ-E2F3的表达量,筛选宫颈癌细胞系,通过qRT-PCR检测宫颈癌细胞系和正常宫颈黏膜上皮细胞中circ-E2F3的表达量。采用CCK-8检测细胞活力,通过检索数据库筛查circ-E2F3可能的分子机制,并通过双荧光素酶分析、qRT-PCR等方法证实其调控机制。结果circ-E2F3在宫颈癌组织和宫颈癌细胞系CaSki中表达上调。体外实验证实circ-E2F3能促进宫颈癌细胞增殖;体内实验证实circ-E2F3通过抑制miR296-5p促进肿瘤细胞生长。结论circ-E2F3通过抑制miR-296-5p在宫颈癌的进展中发挥重要作用,高表达的circ-E2F3可能是判断宫颈癌进展的重要指标。Objective To study the effect of circ-E2F3 on the progression of cervical cancer by inhibiting miR-296-5p.Methods The expression levels of circ-E2F3 in cervical cancer tissues and adjacent tissues were detected by qRT-PCR,and cervical cancer cell lines were then screened.The expression levels of circ-E2F3 expression in cervical cancer cell lines and normal cervical mucosal epithelial cells were detected by qRT-PCR.Cell viability was detected by CCK-8.The possible molecular mechanism of circ-E2F3 was screened by searching the database,and its regulatory mechanism was confirmed by dual luciferase analysis and qRT-PCR.Results The expression of circ-E2F3 was up-regulated in cervical cancer tissues and cervical cancer cell line CaSki.In vitro experiment found that circ-E2F3 can promote the proliferation of cervical cancer cells.circ-E2F3 promoted the tumor growth in vivo by inhibiting miR-296-5p.Conclusion circ-E2F3 played an important role in the progress of cervical cancer by inhibiting miR-296-5p.Highly-expressed circ-E2F3 may be an important indicator to predict the progress of cervical cancer.

关 键 词：circ-E2F3 miR-296-5p 宫颈癌 增殖 

分 类 号：R737.33[医药卫生—肿瘤]