Production of N-acetylglucosamine with Vibrio alginolyticus FA2,an emerging platform for economical unsterile open fermentation  

作　　者：Yuan Peng Ping Xu Fei Tao 

机构地区：[1]State Key Laboratory of Microbial Metabolism,Joint International Research Laboratory of Metabolic and Developmental Sciences,and School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai,People’s Republic of China

出　　处：《Synthetic and Systems Biotechnology》2023年第3期546-554,共9页合成和系统生物技术（英文）

基　　金：the grant from National Natural Science Foundation of China(22138007 and 32170105).

摘　　要：Members of the Vibrionaceae family are predominantly fast-growing and halophilic microorganisms that have captured the attention of researchers owing to their potential applications in rapid biotechnology.Among them,Vibrio alginolyticus FA2 is a particularly noteworthy halophilic bacterium that exhibits superior growth capability.It has the potential to serve as a biotechnological platform for sustainable and eco-friendly open fermentation with seawater.To evaluate this hypothesis,we integrated the N-acetylglucosamine(GlcNAc)pathway into V.alginolyticus FA2.Seven nag genes were knocked out to obstruct the utilization of GlcNAc,and then 16 exogenous gna1s co-expressing with EcglmS were introduced to strengthen the flux of GlcNAc pathway,respectively.To further enhance GlcNAc production,we fine-tuned promoter strength of the two genes and inactivated two genes alsS and alsD to prevent the production of acetoin.Furthermore,unsterile open fermentation was carried out using simulated seawater and a chemically defined medium,resulting in the production of 9.2 g/L GlcNAc in 14 h.This is the first report for de-novo synthesizing GlcNAc with a Vibrio strain,facilitated by an unsterile open fermentation process employing seawater as a substitute for fresh water.This development establishes a basis for production of diverse valuable chemicals using Vibrio strains and provides insights into biomanufacture.

关 键 词：VIBRIO Open fermentation Metabolic engineering N-ACETYLGLUCOSAMINE 

分 类 号：Q81[生物学—生物工程]