蓝光致棕色挪威大鼠慢性视网膜光损伤的实验研究  

Experimental study of chronic retinal damage induced by blue light exposure in Brown Norway rats

作　　者：俞永珍 程天豪 邹秀兰 章梦一 余洋洋 邹玉平 庞龙 YU Yongzhen;CHENG Tianhao;ZOU Xiulan;ZHANG Mengyi;YU Yangyang;ZOU Yuping;PANG Long(Department of Ophthalmology,General Hospital of Southern Theatre Command,Guangzhou 510010,China;Guangzhou University of Traditional Chinese Medicine;the First School of Clinical Medicine,Southern Medical University;Department of Ophthalmology,Guangdong Hospital of Traditional Chinese Medicine)

机构地区：[1]中国人民解放军南部战区总医院眼科,510010 [2]广州中医药大学 [3]南方医科大学第一临床学院 [4]广东省中医院眼科

出　　处：《天津医药》2023年第11期1193-1198,共6页Tianjin Medical Journal

基　　金：广东省自然科学基金项目(2019A1515011732);广州市科技计划项目(202002030413)。

摘　　要：目的通过构建蓝光致棕色挪威(Brown Norway,BN)大鼠慢性视网膜光损伤的模型,探究大鼠光感受器细胞及视网膜色素上皮细胞(RPEc)损伤的特点及可能损伤机制。方法根据随机数字表法将大鼠分组为光照0 d(正常对照组)和光照1、3、7及14 d组,每组8只。正常对照组不进行光照;余各组每日暴露于光照强度为(1000±100)Lux的LED蓝光光源环境中3 h,连续1、3、7及14 d,观察大鼠的行为活动;视网膜电流图(ERG)记录最大混合反应的a、b波振幅和潜伏期;进行眼底照相;HE染色观察视网膜组织;酶联免疫吸附试验(ELISA)检测大鼠视网膜组织的活性氧(ROS)含量。结果与正常对照组比较,光照3 d组对光声刺激的反应迟钝,光照7 d及14 d组精神较萎靡,行动稍迟缓,对光声刺激反应更为迟钝;光照3 d组偶见视网膜出血点,RPEc层基底部色素颗粒增多,外核层可见轻度细胞核固缩;光照7 d组视网膜上见散在点状出血点、黄白色点状颗粒物,视网膜静脉迂曲扩张;光照14 d组视网膜上见大量黄白色点状渗出,视网膜动脉呈铜丝样甚至银丝样外观,视网膜静脉迂曲扩张;光照7、14 d组视网膜光感受器内节/外节结构排列紊乱,外核层细胞核固缩,RPEc层基底部色素颗粒沉积。与正常对照组比较,光照3、7、14 d组大鼠视网膜变薄(P<0.05);光照3、7及14 d组ERG b波潜伏期逐渐延长、a波、b波振幅逐渐降低,视网膜组织ROS逐渐升高(P<0.05)。结论蓝光持续照射BN大鼠可产生氧化应激反应,导致慢性视网膜光损伤,且照射时间越长,视网膜光损伤越重。Objective To investigate the injury characteristics and possible mechanism of photoreceptor cell and retinal pigment epithelium cell(RPEc)by establishing a model of a retinal damage in Brown Norway(BN)rats induced by blue light illumination.Methods According to random number table method,rats were divided into the light 0 d(normal control group),the light 1,3,7 and 14 d groups,with 8 rats in each group.The normal control group was not exposed to light.The other groups were exposed to LED blue light source with light intensity of(1000±100)Lux for 3 h every day for 1,3,7 and 14 days,and the behavior and activities of rats were observed.The amplitudes of a and b waves and latency of the maximum mixed response were recorded by electroretinogram(ERG).Fundus photography was performed.Retinal tissue was observed by HE staining.The content of reactive oxygen species(ROS)in rat retina was detected by enzyme-linked immunosorbent assay(ELISA).Results Compared with the normal control group,the response to photoacoustic stimulation was slow in the 3 d light group,the spirit was weak and the action was slower in the 7 d and 14 d light group,and the response to light and sound was slower.Retinal hemorrhagic spots were occasionally observed in the group treated with 3 days of light irradiation,pigment particles increased in the base of retinal pigment epithelium(RPEc)layer,and mild nuclear pyresis was observed in the outer nuclear layer.In the group exposed to light for 7 days,scattered punctate hemorrhagic spots and yellow-white punctate particles were observed in the retina,and retinal veins were tortuous and dilated.After 14 days of light exposure,a large amount of yellow-white spot-like exudation was observed in retina.The retinal arteries were copper or even silver wire,and retinal veins were tortuous and dilated.The inner segment(IS)/outer segment(OS)of retinal photoreceptors were disordered in the 7 and 14 day light groups,the nucleus of outer nuclear layer shrank,and pigment particles were deposited at the base of RPEc

关 键 词：视网膜色素上皮 创伤和损伤 光感受器细胞 大鼠 近交BN 氧化性应激 蓝光照射 

分 类 号：R774.1[医药卫生—眼科]