lncRNA-AK093407通过EMT促进结直肠癌细胞增殖和转移  

IncRNA-AK093407 Promotes Colorectal Cancer Cell Proliferation and Metastasis Through EMT Progression

作　　者：王建平 王小青[2] 赵学荣[1] 高亚贤[1] 杨岚 肖丽君[1] WANG Jian-ping;WANG Xiao-qing;ZHAO Xue-rong;GAO Ya-xian;YANG Lan;XIAO Li-jun(Department of Immunology,Chengde Medical University,Chengde,Hebei,067000,China;Institute of Chinese Materia Medica,Chengde Medical University)

机构地区：[1]承德医学院免疫学教研室,河北承德067000 [2]承德医学院中药研究所

出　　处：《承德医学院学报》2023年第5期361-366,共6页Journal of Chengde Medical University

基　　金：河北省高等学校科学技术研究项目(QN2016017,QN2020208,ZD2022096);2016年度河北省医学科学研究重点课题计划(20160009);承德医学院基本科研业务费(KY2021024);承德医学院青年基金项目(201831)。

摘　　要：目的研究AK093407对结直肠癌HCT-15细胞的影响及相关机制。方法Lv5慢病毒感染过表达AK093047,转染siRNA沉默AK093407;qRT-PCR鉴定AK093407的表达;MTT和平板克隆形成实验检测细胞增殖;流式细胞术检测细胞周期和凋亡;划痕修复实验和Transwell检测细胞侵袭和迁移;qRT-PCR和蛋白印记检测EMT相关因子。结果Lv5慢病毒感染后,AK093407表达升高。过表达AK093407结直肠癌细胞增殖活性升高,促进细胞周期演进,凋亡降低,迁移和侵袭能力增强。qRT-PCR和蛋白印记结果显示,occludin和E-cadherin的转录和翻译水平降低(P<0.05);β-catenin、vimentin和fibronectin的转录和翻译水平升高(P<0.05)。AK093407沉默后,与上述结果相反。结论AK093407通过EMT促进结直肠癌细胞增殖、细胞周期、侵袭和迁移并抑制细胞凋亡。Objective To study the effect of lncRNA-AK093407(AK093407 for short)on colorectal cancer HCT-15 cells and related mechanisms.Methods Lv5 lentivirus overexpressed AK093047,and transfected siRNA to silence AK093407;qRT-PCR was used to identify the expression of AK093407;MTT and plate colony formation assays were used to detect cell proliferation;flow cytometry was used to detect cell cycle and apoptosis;wound healing assay and transwell was used to detect cell invasion and migration;qRT-PCR and Western blot were used to detect the expression of EMT-related factors.Results The expression of AK093407 increased afer Lv5 lentivirus infection.Overexpression of AK093407 in colorectal cancer cells increased proliferation activity,promoted cell cycle evolution,decreased apoptosis,and enhanced migration and invasion ability.The results of qRT-PCR and Western blotting showed that the transcription and translation levels of occludin and E-cadherin were decreased(P<0.05),whileβ-catenin,vimentin and fibronectin were increased(P<0.05).After the AK093407 gene was silenced,the above results were opposite.Conclusion AK093407 promotes colorectal cancer cell proliferation,cell cycle,invasion and migration and inhibits apoptosis via promoting EMT.

关 键 词：结直肠癌 lnc-AK093407 细胞周期 侵袭 转移 

分 类 号：R735.3[医药卫生—肿瘤]