Efficient scarless skin regeneration enabled by loading micronized amnion in a bioinspired adhesive wound dressing  

作　　者：Huicong Zhang Mingyu Song Changhong Hu Zhiyi Zhang Shuqi Zhang Yan Zhang Yingming Yang Puxu Zhou Liwen Zheng Lan Li Mengjie Mao Yu Shrike Zhang Ping Ji Ximu Zhang 

机构地区：[1]Chongqing Key Laboratory of Oral Disease and Biomedical Sciences and Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education and Stomatological Hospital of Chongqing Medical University,Chongqing,China [2]Division of Engineering in Medicine,Department of Medicine,Brigham and Women’s Hospital,Harvard Medical School,Cambridge,Massachusetts,USA [3]Department of Preventive Dentistry,State Key Laboratory of Oral Diseases,West China Hospital of Stomatology,Sichuan University,Chengdu,China [4]School of Stomatology,Zunyi Medical University,Zunyi,China

出　　处：《Aggregate》2023年第5期96-111,共16页聚集体（英文）

基　　金：National Natural Science Foundation of China,Grant/Award Numbers:32070826,31871464;Science and Technology Research Project of Chongqing Education Commission,Grant/Award Number:KJQN202200471;Chongqing Science and Health Joint Medical,Grant/Award Number:2020GDRC017;CQMU Program for Youth Innovation in Future Medicine,Grant/Award Number:W0075;Senior Medical Talents Program of Chongqing;Key Research Cultivating Project of Stomatological Hospital of Chongqing Medical University,Grant/Award Number:PYZD201603;Program for Innovation Team Building at Institutions of Higher Education in Chongqing in 2016,Grant/Award Number:CXTDG201602006;Brigham Research Institute。

摘　　要：Complete skin reconstruction is a hierarchical,physiological assembly process involving healing of the epidermis,dermis,vasculature,nerves,and cutaneous appendages.To date,few works have reported complete skin regeneration,particularly lacking vascular structures and hair follicles after full skin defects.In this study,a hydrogel derived from the skin secretion of Andrias davidianus(SSAD)that features adhesiveness was used as a bioactive scaffold to load micronized amnion(MA).The SSAD hydrogel was found to promote the migration and proliferation of amnion stem cells and human keratinocytes,as well as inhibit their apoptosis in vitro.In a rat full-skin defect model,the regeneration of skin appendages was observed at the wound area,achieving scarless healing.Transcriptome analyses further validated that SSAD could positively regulate cell migration,proliferation,and differentiation.These functions might be attributed to the abundant growth factors present in the SSAD.Synergized by the delivery of MA,SSAD loaded with the MA could achieve a significantly better skin regeneration effect than SSAD or MA used alone,providing a simple yet highly effective means to obtain complete,scarless skin regeneration,suggesting favorable potential for clinical translation.

关 键 词：AMNION Andrias davidianus cell delivery cutaneous appendages SCARLESS wound dressing 

分 类 号：R318.08[医药卫生—生物医学工程]