Aggregation-induced emission artificial enzyme(AIEzyme)with DNase-like activity:Imaging and matrix cleavage for combating bacterial biofilm  

作　　者：Lei Han Yucui Zhang Baojian Huang Xuhui Bian Ben Zhong Tang 

机构地区：[1]College of Chemistry and Pharmaceutical Sciences,Qingdao Agricultural University,Qingdao,Shandong,China [2]School of Science and Engineering,Shenzhen Institute of Aggregate Science and Technology,The Chinese University of Hong Kong,Shenzhen,Guangdong,China

出　　处：《Aggregate》2023年第5期161-173,共13页聚集体（英文）

基　　金：Youth Innovation Team Project for Talent Introduction and Cultivation in Universities of Shandong Province,Grant/Award Number:096-1622002;Research Foundation for Distinguished Scholars of Qingdao Agricultural University,Grant/Award Number:663-1117015。

摘　　要：DNase-catalyzed hydrolysis of extracellular DNA(eDNA)has been widely employed to eradicate intractable biofilms.Although aggregation-induced emission(AIE)has become the ideal tool for killing planktonic bacteria,AIE luminogens(AIEgens)often lack DNase-mimetic activity,so as to suffer from poor anti-biofilm capacity.Here,an“AIEzyme”,a kind of AIE nanomaterial with enzyme-like activity,is designed and synthesized,where the AIEgens are used as the ligands of Zr-based coordination polymer nanoparticles.Not only does AIEzyme have enduring DNasemimetic activity with low activation energy,but also structural rigidity-stabilized fluorescence.Due to the long-acting hydrolysis for eDNA in biofilm,AIEzyme can efficiently disorganize the established biofilms with good penetrability and realize the healing of superbug-infected wounds under only one dose of AIEzyme.This work provides a strategy to endow ordinary AIEgens with DNase-like and antibiofilm activities.Moreover,AIEzymes can be observed by virtue of their own AIE character,facilitating the study on self-positioning and residual amount of AIEzymes in wounds.The concept“AIEzyme”would hopefully stimulate the tremendous expansion for the tool kits and the application of AIEgens and artificial enzymes.

关 键 词：aggregation-induced emission artificial enzyme BIOFILM DNASE extracellular DNA 

分 类 号：O62[理学—有机化学]