龟鹿二仙胶治疗阿尔茨海默病研究进展及其抗氧化应激的网络药理学分析  

Research Progress of Gulu Erxian Gum in the Treatment of Alzheimer's Disease and its Network Pharmacological Analysis of Anti-oxidative Stress

作　　者：何蕾 郭锦晨 韩辉[3] 宋宇琪 夏泽华 李子龙 HE Lei;GUO Jinchen;HAN Hui;SONG Yuqi;XIA Zehua;LI Zilong(Anhui University of Chinese Medicine,Anhui Hefei 230038,China;Key Laboratory of Xin’an Medicine,Ministry of Education,Anhui Hefei 230038,China;The First Affiliated Hospital of Anhui University of Chinese Medicine,Anhui Hefei 230031,China)

机构地区：[1]安徽中医药大学,安徽合肥230038 [2]新安医学教育部重点实验室,安徽合肥230038 [3]安徽中医药大学第一附属医院,安徽合肥230031

出　　处：《中医药临床杂志》2023年第10期1974-1982,共9页Clinical Journal of Traditional Chinese Medicine

基　　金：安徽中医药大学国家级大学生创新创业项目(G202210369041);国家自然科学基金面上项目(81473534);2022年度安徽省高校科研社科重点项目(2022AH050427);安徽中医药大学2021年人文社科重点项目(2021rwzd22)。

摘　　要：阿尔茨海默病(Alzheimer’s disease,AD)是一种中枢神经退行性疾病,严重危害人类健康。中医在“肾生髓,脑为髓海”的理论指导下,采用补肾生髓法防治痴呆已经有了数千年的历史。龟鹿二仙胶出自明代王三才的《医便》,是补肾生髓法的代表方,具有增强免疫、抗衰老、益智等药理作用,可改善AD患者症状。文章对龟鹿二仙胶治疗AD相关研究进行综述的同时,从氧化应激角度采用网络药理学及分子对接技术建立龟鹿二仙胶治疗AD的“关键活性成分-核心靶点-重要通路”的网络图,对靶点的基因功能和通路进行富集,从而探讨龟鹿二仙胶干预AD氧化应激可能的作用机制。通过TCMSP数据库、BATMAN-TCM数据库以及Herb数据库以及查阅文献确定龟鹿二仙胶的有效成分及对应靶点,经UnitProt数据库查询对应的基因名称,最终得到龟鹿二仙胶活性成分67种活性成分和相应靶基因79个。从GeneCards数据库中筛选出AD和氧化应激的共同靶点6100个,取交集得到龟鹿二仙胶与疾病共有靶点68个。基于DAVID数据库,进行GO功能分析与KEGG通路富集分析,结果表明,龟鹿二仙胶干预AD氧化应激的机制可能是通过等山柰酚、β-谷甾醇和豆甾醇成分作用于IL6、PPARG、CASP3等靶点,从而影响脂质和动脉粥样硬化、化学致癌作用-受体活化、癌症通路、肿瘤坏死因子信号通路等信号通路发挥作用。Alzheimer’s disease(AD)is a central nervous system degenerative disease that seriously endangers human health.Under the guidance of the theory of“kidneys generate marrow,and the brain is the marrow sea”,traditional Chinese medicine has used the method of nourishing the kidneys to generate marrow to prevent and treat dementia for thousands of years.Guilu Erxianjiao comes from the“Yibian”written by WANG Sancai in the Ming Dynasty.It is a representative prescription for tonifying the kidneys and regenerating marrow.It has pharmacological effects such as enhancing immunity,anti-aging,and improving intelligence,and can improve the symptoms of AD patients.While reviewing the relevant research on the treatment of AD by Guilu Erxianjiao,the article also uses network pharmacology and molecular docking technology to establish the“key active ingredients-core targets-important pathways”of Guilu Erxianjiao in the treatment of AD from the perspective of oxidative stress.The network diagram was used to enrich the gene functions and pathways of the target points to explore the possible mechanism of Guilu Erxianjiao in interfering with AD oxidative stress.The active ingredients and corresponding targets of Guilu Erxianjiao were determined through the TCMSP database,BATMAN-TCM database,Herb database and literature review.The corresponding gene names were queried through the UnitProt database,and 67 active ingredients of Guilu Erxianjiao were finally obtained.and 79 corresponding target genes.From the GeneCards database,6,100 common targets for Alzheimer’s disease and oxidative stress were screened out,and 68 common targets between Guilu Erxianjiao and the disease were obtained by intersection.Based on the DAVID database,GO functional analysis and KEGG pathway enrichment analysis were performed.The results showed that the mechanism of Guilu Erxianjiao interfering with AD oxidative stress may be through the action of components such as kaempferol,β-sitosterol and stigmasterol on IL6,PPARG,CASP3 and other targets,thu

关 键 词：龟鹿二仙胶 补肾生髓 阿尔茨海默病 网络药理学 分子对接 氧化应激 

分 类 号：R285[医药卫生—中药学]