Rapid sensitive fluorescence detection of cadmium(Ⅱ)with pyrene excimer switching aptasensor  

作　　者：Hao Yu Qiang Zhao 

机构地区：[1]State Key Laboratory of Environmental Chemistry and Ecotoxicology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]School of Environment,Hangzhou Institute for Advanced Study,UCAS,Hangzhou 310000,China

出　　处：《Journal of Environmental Sciences》2023年第11期1-7,共7页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.22074156 and 21874146).

摘　　要：Heavy metal cadmium(Ⅱ)contamination often occurs,causing great health risk to human due to high toxicity of cadmium(Ⅱ).Rapid,sensitive and simple detection of cadmium(Ⅱ)are of great importance in environmental monitoring.Taking advantage of aptamer in specific recognition,easy modification,and capability of binding-induced structure change,here we reported a simple fluorescent sensor with rapid and sensitive response for Cd^(2+)using aptamer pyrene excimer switch.The aptamer was labeled with dual pyrene molecules at two ends of the sequence.The binding of Cd^(2+)to this aptamer probe brought the pyrene labels into close proximity and enhanced formation of a pyrene excimer,which generated increased fluorescence at 485 nm.By measuring the fluorescence of pyrene excimer,we achieved detection of Cd^(2+)with this aptasensor.Under the optimum experimental conditions,the detection limit of Cd^(2+)reached nanomolar levels.This method was selective and allowed for the detection of Cd^(2+)in tap water.This fluorescence aptasensor is promising for rapid detection of Cd^(2+)in broad applications.

关 键 词：APTAMER CADMIUM Pyrene excimer Fluorescence Sensor 

分 类 号：X832[环境科学与工程—环境工程]