黄芪多糖对脑缺血大鼠顶叶皮质Notch1、Hes1蛋白表达的影响  

Effects of Astragalus polysaccharides on Notch1 and Hes1 protein expression in the parietal cortex of rats with cerebral ischemia

作　　者：李雯娟 吴锋 缪化春 李怀斌 Li Wenjuan;Wu Feng;Miao Huachun;Li Huaibin(School of Basic Medicine,Wannan Medical College,Wuhu 241002,Anhui,China)

机构地区：[1]皖南医学院基础医学院,安徽芜湖241002

出　　处：《右江民族医学院学报》2023年第5期699-703,721,共6页Journal of Youjiang Medical University for Nationalities

基　　金：安徽省高校科学研究重点项目(KJ2020A0603);皖南医学院大学生科研基金项目(WK2022XS45)。

摘　　要：目的观察黄芪多糖对脑缺血大鼠顶叶皮质的神经保护作用,探究其对Notch1、Hes1蛋白表达的影响。方法将48只SD大鼠随机分为正常组、假手术组、模型组、黄芪多糖组,每组12只。复制局灶性脑缺血大鼠模型,黄芪多糖组大鼠采取腹腔注射黄芪多糖50mg/kg,每天1次,连续14d。采用Longa神经功能评分检测各组大鼠治疗前后神经功能缺损情况,Nissl染色法观察脑缺血大鼠顶叶皮质神经元变化情况,免疫组织化学法和WesternBlot法检测大鼠缺血侧顶叶皮质Notch1和Hes1蛋白的表达情况。结果与假手术组相比,模型组大鼠神经功能评分升高(P<0.001),顶叶皮质区神经细胞轮廓模糊,部分细胞呈空泡状,核固缩,尼氏阳性神经元数量减少(P<0.001),Notch1与Hes1蛋白表达升高(P<0.01)。与模型组相比,黄芪多糖组大鼠神经功能评分降低(P<0.05),细胞形态趋于正常,尼氏阳性神经元数量增多(P<0.01)。Notch1和Hes1蛋白表达降低(P<0.05)。结论黄芪多糖能够对脑缺血大鼠顶叶皮质神经损伤起到保护作用,其机制可能与抑制Notch1、Hes1过表达有关。Objective To observe the neuroprotective effects of Astragalus polysaccharides(APS)in the parietal cortex of rats with cerebral ischemia,and to explore its impact on the expression of Notch1 and Hes1 proteins.Methods Forty-eight SD rats were randomly divided into the normal,sham operation,model,and APS groups,with 12 rats in each group.A focal cerebral ischemia model was replicated.Rats in the APS group received intraperitoneal injections of APS(50 mg/kg)once a day for 14 consecutive days.The Longa nerve function score was used to assess neurological impairment of rats in each group before and after treatment,Nissl staining was used to observe changes in parietal cortex neurons of rats with cerebral ischemia.Immunohistochemistry and Western Blot were used to analyze Notch1 and Hes1 protein expression in the ischemic parietal cortex of rats.Results Compared with the sham operation group,rats in the model group showed an increase in the neurological function score(P<0.001),blurred outlines of nerve cells in the parietal cortex,vacuolar changes in some cells,karyopyknosis,and a decrease in Nissl-positive neuron count(P<0.001).The expression of Notch1 and Hes1 proteins was elevated(P<0.01).Compared with the model group,rats in the APS group exhibited a decrease in the neurological function score(P<0.05),the morphology of cells tended toward normal,and an increase in the number of Nissl-positive neurons(P<0.01).Notch1 and Hes1 protein expression decreased(P<0.05).Conclusion Astragalus polysaccharides provide protective effects against nerve injury in the parietal cortex of rats with cerebral ischemia,possibly through the inhibition of Notch1 and Hes1 overexpression.

关 键 词：黄芪多糖 脑缺血 顶叶皮质 NOTCH1 HES1 

分 类 号：R285[医药卫生—中药学]