黏结蛋白聚糖2基因多态性与结直肠癌的关联及其基因型频率分析  

Association of syndecan-2 gene polymorphism in colorectal cancer and genotype frequency analysis

作　　者：宋新江[1] 韩利江[1] 张利萍[1] 孙国钢[1] 俞斌[1] 劳玲娟[1] Song Xinjiang;Han Lijiang;Zhang Liping;Sun Guogang;Yu Bin;Lao Lingjuan(Anorectal Surgery Department,General Medical Community Hospital of Shaoxing Second Hospital,Shaoxing 312000,China)

机构地区：[1]绍兴第二医院医共体总院肛肠外科,绍兴312000

出　　处：《中华全科医师杂志》2023年第10期1073-1076,共4页Chinese Journal of General Practitioners

基　　金：2020年柯桥区社发类经费自筹科技计划(2020KZ59)。

摘　　要：该研究为横断面研究,入选2020年5月5日至2022年5月28日绍兴第二医院医共体总院收治的结直肠癌患者225例作为结直肠癌组,同期选取来该院进行结直肠健康检查的健康人101人作为健康对照组。记录结直肠癌患者的年龄和性别,通过组织活检确认结直肠癌临床分期,根据CT扫描技术判定淋巴结转移情况、肿瘤直径、病灶数等。记录健康对照人群的性别、年龄,经结直肠组织活检确认无相关疾病。通过结肠镜获取入选者的病理组织,所有组织标本采用Sanger测序确定黏结蛋白聚糖(SDC)基因多态性位点。采用单因素logistic回归模型分析SDC单核苷酸多态性与结直肠癌的关联。结果显示,结直肠癌组患者中临床Ⅲ/Ⅳ期[120例(53.3%)]、有淋巴结转移[126例(56.0%)]、肿瘤直径<2 cm[170例(75.6%)]、单发肿瘤[193例(85.8%)]者占比较高。单因素logistic回归分析结果显示SDC2 rs2515127位点的基因多态性与结直肠癌存在关联(OR=1.643,95%CI:1.025~2.337,P=0.012)。根据密码子AGC突变情况将SDC2 rs2515127位点分为AA、AG、GG 3种基因型,本组结直肠癌患者GG基因型频率最高,为60.7%(102/168),AG、AA基因型频率分别为30.4%(51/168)、8.9%(15/168)。该研究结果显示SDC2 rs2515127位点的基因多态性与结直肠癌的存在关联,且SDC2 rs2515127位点的基因型中GG和AG基因型频率较高。A total of 225 patients with colorectal cancer(CRC)admitted to the General Hospital of Shaoxing Second Hospital Medical community from May 5,2020 to May 28,2022 were enrolled(CRC group),and 101 healthy subjects underwent colorectal examination were selected as the control group.The tissue biopsy samples of all subjects were obtained by colonoscopy,and subjected to Sanger sequencing to determine the polymorphism sites of the syndecan-2(SDC2)gene.The association between SDC single nucleotide polymorphism(SNP)and colorectal cancer in CRC patients was analyzed with logistic regression.The logistic regression analysis showed that the gene polymorphism of SDC2 rs2515127 was associated with colorectal cancer(OR=1.643,95%CI:1.025-2.337,P=0.012).The frequency of GG,AG and AA the in genotypes of SDC2 rs2515127 was 60.7%(102/168),30.4%(51/168)and 8.9%(15/168),respectively.The results showed that the gene polymorphism of SDC2 rs2515127 was associated with colorectal cancer,and the frequencies of GG and AG genotypes were higher in the genotypes of SDC2 rs2515127.

关 键 词：结直肠肿瘤 黏结蛋白聚糖2 多态性 单核苷酸 

分 类 号：R735.34[医药卫生—肿瘤]