Use of recombinant microRNAs as antimetabolites to inhibit human non-small cell lung cancer  

作　　者：Yixin Chen Mei-Juan Tu Fangwei Han Zhenzhen Liu Neelu Batra Primo N.Lara Hong-Wu Chen Huichang Bi Ai-Ming Yu 

机构地区：[1]Department of Biochemistry and Molecular Medicine,School of Medicine,UC Davis,Sacramento,CA 95817,USA [2]School of Pharmaceutical Sciences,Sun Yat-sen University,Guangzhou 510006,China [3]School of Public Health,UNT Health Science Center,Fort Worth,TX 76107,USA [4]Department of Internal Medicine,School of Medicine,UC Davis,Sacramento,CA 95817,USA

出　　处：《Acta Pharmaceutica Sinica B》2023年第10期4273-4290,共18页药学学报（英文版）

基　　金：supported in part by the National Cancer Institute[R01CA225958,R01253230];National Institute of General Medical Sciences[R35GM140835];National Institutes of Health(USA);supported by grants from the Chinese National Key Research and Development Program(2017YFE0109900,China);supported by a scholarship from the Chinese Scholarship Council(No.201806380133,China);the Molecular Pharmacology,Flow Cytometry,and Mouse Biology Shared Resources funded by the UC Davis Comprehensive Cancer Center Support Grant awarded by the National Cancer Institute(P30CA093373),National Institutes of Health。

摘　　要：During the development of therapeutic microRNAs(miRNAs or miRs),it is essential to define their pharmacological actions.Rather,miRNA research and therapy mainly use miRNA mimics synthesized in vitro.After experimental screening of unique recombinant miRNAs produced in vivo,three lead antiproliferative miRNAs against human NSCLC cells,miR-22-3p,miR-9-5p,and miR-218-5p,were revealed to target folate metabolism by bioinformatic analyses.Recombinant miR-22-3p,miR-9-5p,and miR-218-5p were shown to regulate key folate metabolic enzymes to inhibit folate metabolism and subsequently alter amino acid metabolome in NSCLC A549 and H1975 cells.Isotope tracing studies further confirmed the disruption of one-carbon transfer from serine to folate metabolites by all three miRNAs,inhibition of glucose uptake by miR-22-3p,and reduction of serine biosynthesis from glucose by miR-9-5p and-218-5p in NSCLC cells.With greater activities to interrupt NSCLC cell respiration,glycolysis,and colony formation than miR-9-5p and-218-5p,recombinant miR-22-3p was effective to reduce tumor growth in two NSCLC patient-derived xenograft mouse models without causing any toxicity.These results establish a common antifolate mechanism and differential actions on glucose uptake and metabolism for three lead anticancer miRNAs as well as antitumor efficacy for miR-22-3p nanomedicine,which shall provide insight into developing antimetabolite RNA therapies.

关 键 词：RNA therapy Folate metabolism Amino acid GLYCOLYSIS miR-22 MIR-9 MIR-218 Lung cancer 

分 类 号：R96[医药卫生—药理学]