BDE-47降解基因工程菌的构建及降解效应研究  

Construction and Degradation Effect of a BDE-47 Aerobically Degradable Genetically Engineered Bacteria

作　　者：田婧 王继华[1] TIAN Jing;WANG Jihua(Institute of Life Science and Technology,Harbin Normal University,Harbin 150025,China)

机构地区：[1]哈尔滨师范大学生命科学与技术学院,黑龙江哈尔滨150025

出　　处：《环境科学与技术》2023年第7期8-14,共7页Environmental Science & Technology

基　　金：2022年度黑龙江省省属高等学校基本科研业务费科研项目(2021-KYYWF-0172)。

摘　　要：为提高BDE-47好氧降解菌的降解效率和环境适应性,作者以实验室前期研究的BDE-47好氧降解菌GB-2为供体菌,以AntABC基因簇为目的基因,使用从电子垃圾污染的土壤中筛选的土著优势菌WT-G为受体菌,构建了一株基因工程菌,并置于BDE-47污染土壤中开展模拟降解试验。结果表明:BDE-47好氧降解基因工程菌可稳定遗传,传代30代的菌株降解率可稳定保持在72%~76%之间。在100μg/L的BDE-47中,基因工程菌的BDE-47降解率(76.78%)高于供体菌GB-2(70.17%);在1500 ng/g的BDE-47污染土壤中,基因工程菌的BDE-47降解率(47.15%)也高于供体菌GB-2(41.98%)。BDE-47好氧降解基因工程菌的降解效果优于供体菌GB-2。BDE-47好氧降解基因工程菌降解能力较稳定,且对污染环境具有较好适应性。To improve the degradation efficiency and environmental adaptability of BDE-47 aerobic degrading bacteria,a genetically engineered bacterium was constructed using the BDE-47 aerobic degrading bacteria GB-2 studied in the laboratory as the donor bacteria and the AntABC gene cluster as the target gene,using the indigenous dominant bacteria WT-G screened from the soil contaminated by e-waste as the recipient bacteria.Simulated degradation test placed in BDE-47 contaminated soil.The results showed that BDE-47 aerobically degradable genetically engineered bacteria could be stably inherited,the degradation rate of the strain could be stably maintained between 72%~76%after 30 generations.In 100μg/L of BDE-47,the degradation rate of BDE-47 in genetically engineered bacteria(76.78%)was higher than that of donor bacteria GB-2(70.17%).In BDE-47 contaminated soil at 1500 ng/g,the BDE-47 degradation rate(47.15%)of genetically engineered bacteria was also higher than that of donor bacteria GB-2(41.98%).The degradation effect of BDE-47 aerobically degradable genetically engineered bacteria was better than that of donor bacteria GB-2.BDE-47 aerobic degradation genetic engineering bacteria have stable degradation ability and good adaptability to polluted environment.

关 键 词：BDE-47降解 土著优势菌 基因工程菌 土壤 

分 类 号：X172[环境科学与工程—环境科学] X592