Network pharmacology and preliminary cell screening studies on the anti-liver cancer activity of Nauclea Officinalis  

作　　者：CHEN Wei-jia ZHOU Ming-yan HU Ji-cheng ZHU Ze XU Jian 

机构地区：[1]Department of Organ Transplantation,the Second Affiliated Hospital of Hainan Medical University,Haikou 570105,China [2]Institute of Clinical Medicine,the Second Affiliated Hospital of Hainan Medical University,Haikou 570105,China [3]Hainan Institute of Organ Transplantation,Haikou 570105,China

出　　处：《Journal of Hainan Medical University》2023年第12期1-9,共9页海南医学院学报(英文版)

基　　金：Major Science and Technology Project of Hainan Province(No.ZDKJ2019009);Construction Project of Clinical Medical Center in Hainan Province。

摘　　要：Objective:To explore the mechanism of Nauclea Officinalis of anti-liver cancer effect based on network pharmacology,and to preliminarily verify anti-liver cancer activity of Nauclea Officinalis through cell screening.Methods:Network pharmacology was used to screen for common targets of Nauclea Officinalis and liver cancer,protein-protein interaction(PPI)network was constructed,and enrichment analysis and mechanism prediction were conductd.Molecular docking of main active ingredients of Nauclea Officinalis with core targets was made.Preliminary verification was performed by in vitro cell experiments such as CCK8,cell apoptosis,and PCR.Results:After the screening,14 active ingredients of Nauclea Officinalis were obtained,with 587 related targets.After mapping with liver cancer targets,there were 288 common targets,mainly including TP53,SRC,STAT3,and other core targets.Among them,compounds such as strictosamide,pumiloside and vincosamide may be potential active ingredients of Nauclea Officinalis of anti-liver cancer effect.They may participate in protein phosphorylation and negative regulation of the apoptosis process by mediating cancer pathways,PI3K/Akt and EGFR tyrosine kinase inhibitors resistance signaling pathways to play an anti-liver cancer role;molecular docking results showd that active ingredients of Nauclea Officinalis had a stable binding with liver cancer core targets;in vitro cell experiments showd that main ingredient strictosamide of Nauclea Officinalis had cytotoxicity against liver cancer cells,inhibited liver cancer cell proliferation(P<0.001),down-regulated gene expression of liver cancer HepG2 cells SRC,STAT3,MAPK3(P<0.05),and induced liver cancer cell apoptosis(P<0.001).Conclusion:This study preliminarily explores the potential mechanism of active ingredients of Nauclea Officinalis against liver cancer and its preliminary pharmacological effects,providing a theoretical basis for the study of Nauclea Officinalis of anti-liver cancer mechanism.

关 键 词：Nauclea Officinalis HEPATOCARCINOMA Network pharmacology Mechanism of action In vitro cell assay 

分 类 号：R285[医药卫生—中药学]