玉米须不同萃取物的细胞毒性及对痴呆小鼠的治疗作用  

Cytotoxicity of different extracts of Stigma Maydis and their therapeutic effect on dementia mice

作　　者：朱小婷 郭江涛 刘杰 简梨娜 陶添泽 ZHU Xiaoting;GUO Jiangtao;LIU Jie;JIAN Lina;TAO Tianze(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China)

机构地区：[1]贵州中医药大学,贵州贵阳550025

出　　处：《贵州科学》2023年第4期31-37,共7页Guizhou Science

基　　金：贵州省科技基金(黔科合基础[2018]1013);国家自然科学基金委员会-贵州省人民政府联合基金(No.U1812403-2);黔科合基础-ZK[2022]一般463;黔教合YJSKYJJ[2021]164。

摘　　要：目的:观察玉米须不同萃取物石油醚组(PF)、二氯甲烷组(DF)、乙酸乙酯组(AF)、正丁醇组(BF)对神经细胞活性的影响,并探讨其对老年痴呆小鼠的治疗作用。方法:MTT法测定玉米须不同萃取物对PC12及U87两种神经细胞存活率的影响;构建老年痴呆小鼠模型,采用Morris水迷宫试验与跳台试验检测小鼠认知与学习记忆能力;ELISA法检测小鼠血液中IL-1β、TNF-α、T-SOD、MDA以及脑中T-SOD和MDA的含量。结果:玉米须萃取物AF、BF组对PC12细胞有增殖作用,具有一定的神经营养功能;PF、DF、AF组对U87细胞的增殖具有一定的抑制作用;跳台实验、水迷宫实验和空间探索实验结果表明玉米须各萃取物组均能改善痴呆小鼠的空间记忆及学习能力;小鼠海马CA1区的切片结果表明玉米须各萃取物组可以维持痴呆小鼠脑组织结构形态完整,BF组疗效最佳;ELISA法检测结果表明玉米须各萃取物组均能提高小鼠血清中T-SOD活力,降低MDA含量及TNF-α和IL-1β的表达,其中AF组、BF组及PF组效果较为明显;小鼠脑组织中T-SOD及MDA含量实验结果表明AF组、BF组效果较好,说明玉米须各萃取物组可以改善痴呆小鼠脑组织氧化应激的损伤。结论:玉米须不同萃取部位可改善老年痴呆小鼠模型的认知功能,综合所测各项指标,得到的治疗效果为:BF>AF>PF>DF。The effect of Stigma Maydis extracts extracted by petroleum ether(PF),dichloromethane(DF),ethyl acetate(AF) and n-butanol(BF) on the activity of nerve cells was observed,and their therapeutic effect on senile dementia mice was explored.MTT method was used to determine the effect of different extracts of Stigma Maydis on the survival rate of PC12 and U87 nerve cells.Morris water maze test and diving platform test were used to detect the cognition,learning and memory ability of dementia mice.The contents of IL-1β,TNF-α,T-SOD and MDA in the blood and brain of the mice were determined by ELISA method.The results showed that AF and BF groups had proliferation effect on PC12 cells,and had certain neurotrophic function;PF,DF and AF groups could inhibit the proliferation of U87 cells to a certain extent.The results of diving platform experiment,water maze experiment and space exploration experiment showed that each extract group could improve the spatial memory and learning ability of dementia mice.Section results of hippocampal CA1 region of mice showed that each extract group could maintain the integrity of brain tissue structure of dementia mice,and BF group had the best effect.The results of ELISA method showed that each extract group could increase the activity of T-SOD,decrease the content of MDA and the expression of TNF-α and IL-1β in serum of mice,among which the effect of AF,BF and PF groups was more obvious.The experimental results of T-SOD and MDA content in brain tissues of mice showed that AF and BF groups had better effect,indicating that each extract group could improve the oxidative stress damage of brain tissues of dementia mice.In conclusion,different extracts of Stigma Maydis can improve the cognitive function of senile dementia mice,and the therapeutic effect is BFAFPFDF.

关 键 词：玉米须 MTT法 老年痴呆 水迷宫 跳台实验 

分 类 号：R285.5[医药卫生—中药学]