溪黄草对circ_0091579/miR-515-5p、肺癌细胞A549增殖及凋亡的影响  

Effect of rabdosia lophanthoides on circ_0091579/miR-515-5p and proliferation and apoptosis of lung cancer cell A549

作　　者：何苗 姚文秀[2] 谷涉群[3] HE Miao;YAO Wenxiu;GU Shequn(Health Management Center,the Third Hospital of Mianyang,Sichuan Provincal Center for Mental Health,Mianyang 621000,Sichuan,China;Department of Chest Oncology,Sichuan Cancer Hospital,Chengdu 610041,Sichuan,China;Department of Oncology,Chenzhou First People s Hospital,Chenzhou 423099,Hunan,China)

机构地区：[1]绵阳市第三人民医院,四川省精神卫生中心健康管理中心,四川绵阳621000 [2]四川省肿瘤医院胸部肿瘤内科,四川成都610041 [3]郴州市第一人民医院肿瘤内科,湖南郴州423099

出　　处：《中南医学科学杂志》2023年第5期660-664,共5页Medical Science Journal of Central South China

基　　金：吴阶平医学基金会胸腹部肿瘤精准治疗燃石检测专项资助基金(320675018536)。

摘　　要：目的探讨溪黄草对circ_0091579/miR-515-5p及肺癌细胞A549增殖和凋亡的影响。方法将肺癌A549细胞分为对照组、溪黄草(20、40、80 mg/L)组、si-circ_0091579组、si-NC组、pcDNA-circ_0091579组、pcDNA组、溪黄草+pcDNA-circ_0091579组。CCK-8、平板克隆实验、流式细胞术检测A549细胞抑制率、克隆形成数以及凋亡率。qRT-PCR检测circ_0091579和miR-515-5p表达。双荧光素酶报告实验验证circ_0091579和miR-515-5p相互作用。结果溪黄草显著降低A549细胞的增殖和克隆形成能力,促进细胞凋亡,诱导cleaved-Caspase-3表达上调及circ_0091579下调,且呈质量浓度依赖性。下调circ_0091579可降低A549细胞的增殖和克隆形成能力,促进细胞凋亡,诱导cleaved-Caspase-3表达上调。上调circ_0091579可逆转溪黄草对A549细胞的增殖抑制和凋亡促进功能。circ_0091579和miR-515-5p直接特异性结合。结论溪黄草可抑制肺癌细胞A549增殖,诱导其凋亡,其机制与下调circ_0091579/miR-515-5p途径有关。Aim To investigate the effect of Rabdosia lophanthoides on circ_0091579/miR-515-5p and proliferation and apoptosis of lung cancer cell A549.Methods Lung cancer A549 cells were divided into control group,Rabdosia lophanthoides(20,40,80 mg/L)group,si-circ_0091579 group,si-NC group,pcDNA-circ_0091579 group,pcDNA group,Rabdosia lophanthoides+pcDNA-circ_0091579 group.CCK-8,plate cloning experiment and flow cytometry were performed to evaluate the inhibition rate,clone formation number and apoptosis rate of A549 cells.qRT-PCR was used to detect circ_0091579 and miR-515-5p expression.The interaction between circ_0091579 and miR-515-5p was verified via dual-luciferase reporter assays.Results Rabdosia lophanthoides significantly reduced the proliferation and clonogenicity of A549 cells,promoted cell apoptosis,induced up-regulation of cleaved-Caspase-3 expression and decreased circ_0091579 level in a mass concentration dependent manner.Down-regulation of circ_0091579 reduced the proliferation and clonogenicity of A549 cells,promoted cell apoptosis,and induced up-regulation of cleaved-Caspase-3 expression.Up-regulation of circ_0091579 could reverse the proliferation inhibition and apoptosis promotion of A549 cells.circ_0091579 directly and specifically bound to miR-515-5p.Conclusion Rabdosia lophanthoides inhibited the proliferation and induced apoptosis of lung cancer cell A549,which was associated with the down-regulation of circ_0091579/miR-515-5p pathway.

关 键 词：溪黄草 肺癌 circ_0091579 miR-515-5p 增殖 凋亡 

分 类 号：R734.2[医药卫生—肿瘤]