miR-199a-3p靶向CD151对甲状腺癌B-CPAP细胞增殖、凋亡、侵袭和上皮间质转化的影响  

Effects of miR-199a-3p Targeting CD151on Proliferation,Apoptosis,Invasion and Epithelial-mesenchymal Transformation of B-CPAP Thyroid Cancer Cells

作　　者：覃艳 赵可雷[2] 李燕[1] 朱云峰[1] 曹萌[1] Qin Yan;Zhao Kelei;Li Yan(Department of Endocrinology,the First Affiliated Hospital of Xinxiang Medical University,Xinxiang 453100,China;Department of Oncology,the First Affiliated Hospital of Xinxiang Medical University,Xinxiang 453100,China)

机构地区：[1]新乡医学院第一附属医院内分泌科二病区,新乡453100 [2]新乡医学院第一附属医院肿瘤内科二病区,新乡453100

出　　处：《华中科技大学学报（医学版）》2023年第5期610-616,共7页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：河南省医学科技攻关计划(联合共建)项目(No.LHGJ20190461)。

摘　　要：目的探究miR-199a-3p过表达靶向CD151对甲状腺癌B-CPAP细胞增殖、凋亡、侵袭和上皮间质转化的影响。方法通过双荧光素酶报告基因实验验证miR-199a-3p和CD151的靶向关系。构建CD151pcDNA载体过表达CD151,将miR-199a-3p mimic与pcDNA-CD151单独或联合转染至B-CPAP细胞,将细胞随机分为4组:Control组、miR-199a-3p mimic组、pcDNA-CD151组和miR-199a-3p mimic+pcDNA-CD151组。BrdU染色检测细胞增殖水平;流式细胞术检测细胞凋亡率;流式分选检测细胞周期分布;Transwell检测细胞侵袭能力;Western blot检测MMP-2、Ki-67、Cyclin D1、E-cadherin、N-cadherin、Vimentin蛋白表达。结果CD151与miR-199a-3p存在靶向作用关系。与Control组比较,miR-199a-3p mimic组BrdU阳性细胞数降低,细胞凋亡率升高,G2/M、G1期比例降低,S期比例升高,侵袭细胞数降低,MMP-2、Ki-67、Cyclin D1蛋白表达降低,E-cadherin蛋白表达升高,N-cadherin、Vimentin蛋白表达降低;pcDNA-CD151组BrdU阳性细胞数升高,细胞凋亡率降低,G2/M、G1期比例升高,S期比例降低,侵袭细胞数升高,MMP-2、Ki-67、Cyclin D1蛋白表达升高,E-cadherin蛋白表达降低,N-cadherin、Vimentin蛋白表达升高。与pcDNACD151组比较,miR-199a-3p mimic+pcDNA-CD151组BrdU阳性细胞数降低,细胞凋亡率升高,G2/M、G1期比例降低,S期比例升高,侵袭细胞数降低,MMP-2、Ki-67、Cyclin D1蛋白表达降低,E-cadherin蛋白表达升高,N-cadherin、Vimentin蛋白表达降低(均P<0.01)。结论miR-199a-3p过表达靶向CD151抑制甲状腺癌B-CPAP细胞增殖、侵袭和上皮间质转化,并诱导细胞凋亡。Objective To investigate the effects of miR-199a-3p overexpression targeting CD151on the proliferation,apoptosis,invasion and epithelial-mesenchymal transformation of B-CPAP thyroid cancer cells.Methods The luciferase reporter assay was used to detect the targeting relationship between miR-199a-3p and CD151.CD151pcDNA vector was constructed in which CD151was overexpressed.miR-199a-3p mimic and pcDNA-CD151were transfected into B-CPAP cells,and the cells were randomly divided into four groups:Control group,miR-199a-3p mimic group,pcDNA-CD151group,and miR-199a-3p mimic+pcDNA-CD151group.BrdU staining was used to detect the number of BrdU positive cells.The apoptosis rate was detected by flow cytometry.Cell cycle distribution was detected by flow cytometry.Transwell assay was used to detect the number of invaded cells.The protein expressions of MMP-2,Ki-67,Cyclin D1,E-cadherin,N-cadherin and Vimentin were detected by Western blotting.Results CD151had a targeted interaction with miR-199a-3p.Compared with the Control group,the number of BrdU positive cells in miR-199a-3p mimic group was decreased.The apoptosis rate was increased.The proportions of G2/M and G1 phase were decreased,while the proportion of S phase was increased.The number of invaded cells was decreased,the expressions of MMP-2,Ki-67and Cyclin D1protein were decreased.The expression of E-cadherin protein was increased,yet the expressions of N-cadherin and Vimentin were decreased.In pcDNA-CD151group,the number of BrdU positive cells was increased,the apoptosis rate was decreased,the proportions of G2/M and G1phase were increased,the proportion of S phase was decreased,the number of invaded cells was increased,the expression of MMP-2,Ki-67and Cyclin D1protein were increased,the expression of E-cadherin protein was decreased,and the expressions of N-cadherin and Vimentin were increased.Compared with pcDNA-CD151group,the number of BrdU positive cells in miR-199a-3p mimic+pcDNA-CD151group was decreased,the apoptosis rate was increased,the proportions of G2/M and

关 键 词：甲状腺癌 miR-199a-3p CD151 凋亡 侵袭 上皮间质转化 

分 类 号：R736.1[医药卫生—肿瘤]