IgG Fc段结合蛋白(FCGBP)作为低级别胶质瘤预后标志物的可能性及与免疫浸润的相关性分析  

IgG Fc binding protein(FCGBP)as a prognostic marker of low-grade glioma and its correlation analysis with immune infiltration

作　　者：刘乔 张佳瑞 张富琴 张伟 巩丽 LIU Qiao;ZHANG Jiarui;ZHANG Fuqin;ZHANG Wei;GONG Li(Department of Pathology,The Second Affiliated Hospital of Air Force Medical University,Xi'an 710038,China)

机构地区：[1]空军军医大学第二附属医院病理科,陕西西安710038

出　　处：《细胞与分子免疫学杂志》2023年第8期686-692,共7页Chinese Journal of Cellular and Molecular Immunology

基　　金：唐都医院创新基金(2018LCYJ013)。

摘　　要：目的 探讨IgG Fc段结合蛋白(FCGBP)作为低级别胶质瘤(LGG)预后标志物的可能性及与免疫浸润的相关性。方法 利用癌症基因组图谱(TCGA)、基因型组织表达(GTEX)和中国胶质瘤基因组图谱(CGGA)数据库分析FCGBP在泛癌中的表达情况;选择GSE15824和GSE68848数据集进行验证;利用基因表达谱交互分析(GEPIA)数据库和R语言分析FCGBP与生存预后的关系;基因注释工具Metascape和基因子集富集分析(GSEA)进行功能注释和富集分析;最后利用肿瘤免疫浸润评分(TIMER)数据库分析FCGBP基因在LGG免疫微环境中的表达及与免疫细胞的相关性。结果 FCGBP在LGG组织中高表达,FCGBP高表达提示LGG患者预后不良,受试者工作特征(ROC)曲线分析和多因素风险比例回归模型(COX)分析表明FCGBP是LGG预后的独立危险因素;基因本体论(GO)分析表明,FCGBP参与细胞代谢、定位、正反向调节生物过程,同时参与生物黏附,对病毒及微生物刺激进行应答,参与炎症反应;GSEA通路富集分析表明,FCGBP与Janus激酶/信号转导子与转录激活子(JAK/STAT)通路、 Toll样受体(TLR)通路、趋化因子通路和P53通路均具有显著相关性;FCGBP的表达与LGG免疫微环境中大多数免疫细胞的表达正相关。结论 LGG中FCGBP高表达是患者生存预后的危险因素,且与免疫细胞表达正相关。Objective To identify the possibility of IgG Fc binding protein(FCGBP)acting as a prognostic marker of low-grade glioma(LGG)and its correlation with immune infiltration.Methods The expression of FCGBP was analyzed in pan-cancer using The Cancer Genome Atlas(TCGA),Genotypic tissue expression(GTEX),and China Glioma Genome Atlas(CGGA)database.Then,GSE15824 and GSE68848 datasets were selected for further verification.And gene expression Profile Interaction analysis(GEPIA)database and R language were used to analyze the relationship between FCGBP and survival prognosis.Metascape and GSEA were used for functional annotation and enrichment analysis.Finally,the expression of FCGBP gene in LGG immune microenvironment and its correlation with immune cells were analyzed by TIMER database.Results FCGBP was highly expressed in LGG tissues,indicating poor prognosis of LGG patients.Receiver operating characteristic(ROC)curve analysis and COX analysis showed that FCGBP was an independent risk factor for the prognosis of LGG.Moreover,Gene Ontology(GO)demonstrated that FCGBP was involved in cell metabolism,localization,positive,and negative regulation of biological processes,as well as biological adhesion,response to viral and microbial stimulation,and inflammation.GSEA pathway enrichment analysis showed that FCGBP was significantly correlated with Janus kinase/signal transducer and activator of transcription(JAK/STAT)pathway,Toll-like receptor(TLR)pathway,chemokine pathway,and P53 pathway.In addition,FCGBP expression was positively correlated with the expression of most immune cells in the immune microenvironment of LGG.Conclusion The high expression of FCGBP in LGG is a risk factor for survival and prognosis,and it is positively correlated with the expression of immune cells.

关 键 词：IgG Fc段结合蛋白(FCGBP) 低级别胶质瘤 总生存期 免疫浸润 

分 类 号：R739.41[医药卫生—肿瘤] R730.7[医药卫生—临床医学] R392.12[生物学—生物工程] Q811.4