光信号对AmRosea1过表达84K杨花青素合成及基因表达的影响  

Effects of Light Signal on Anthocyanin Biosynthesis and Gene Expression of AmRosea1 Overexpressed 84K Poplar

作　　者：闫惠铃 张鑫鑫[1] 赵曦阳 曲冠证[1] 王兆宁 韩锐 YAN Huiling;ZHANG Xinxin;ZHAO Xiyang;QU Guanzheng;WANG Zhaoning;HAN Rui(State Key Laboratory of Forest Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040;College of Forestry and Grass-land,Jilin Agriculture University,Changchun 130118)

机构地区：[1]林木遗传育种国家重点实验室,东北林业大学,哈尔滨150040 [2]林学与草学学院,吉林农业大学,长春130118

出　　处：《植物研究》2023年第6期815-825,共11页Bulletin of Botanical Research

基　　金：国家科技创新2030重大课题(2022ZD0401504)。

摘　　要：为了探究光信号引起AmRosea1过表达84K杨(Populus alba×P.glandulosa‘84K’)植株颜色变化的原因,以野生型和AmRosea1过表达84K杨为试验材料,开展LED光、自然光、LED红光、LED蓝光和LED红蓝光处理,测定不同光强和光质下野生型和转基因株系的生理指标变化情况。结果显示:在不同光强下,与LED光处理相比,自然光处理下转基因株系叶片变红,花青素和可溶性糖含量升高,叶绿素含量和POD活性降低。不同光质处理30 d后,与LED红光和蓝光相比,在LED红蓝光诱导下,转基因株系叶片变红,且花青素含量升高,可溶性糖含量和POD活性下降,叶绿素含量略有降低。根据试验结果推测,强光和红蓝光质能激活AmRosea1过表达84K杨的花青素生物合成通路,使转基因株系积累大量的花青素,从而使植株叶片变红。To explore the reasons of color change in AmRosea1 overexpressed 84K poplar(Populus alba×P.glandulosa‘84K’)caused by light signal,the wild type and AmRosea1 overexpressed 84K poplar were used as the materials.The anthocyanin content,chlorophyll content,soluble sugar content and POD activity under LED light,natural light,LED red light,LED blue light,and LED red and blue light treatment were measured,and the changes of physiological indexes of wild type and transgenic lines under different optical signal were determined respectively.The results showed that under different light intensities,compared with the LED light treatment,the leaves of the transgenic lines turned red,and the anthocyanin content and soluble sugar content increased,and the chlorophyll content and POD activity decreased under natural light.After 30 days of different light treatments,the LED red-blue light treatment that the antho-cyanin content increased,the soluble sugar content and POD activity decreased,and the chlorophyll content decreased slightly under LED red and blue light induction compared with LED red and blue light.Based on the experimental results,it was speculated that strong light and red and blue light quality might activate the anthocyanin biosynthesis pathway and accumulate a large amount of anthocyanins in AmRosea1 overexpressed 84K poplar,which in turn the leaves turned red.

关 键 词：光强 光质 AmRosea1 84K杨 花青素 基因表达 

分 类 号：S792.11[农业科学—林木遗传育种]