The piRNA pathway is required for nucleopolyhedrovirus replication in Lepidoptera  

作　　者：Junming Xia Shigang Fei Hongyun Wu Yifan Yang Wensheng Yu Mengmeng Zhang Yiyao Guo Luc Swevers Jingchen Sun Min Feng 

机构地区：[1]Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding,College of Animal Science,South China Agricultural University,Guangzhou,China [2]Insect Molecular Genetics and Biotechnology,National Center for Scientific Research Demokritos,Institute of Biosciences and Applications,Athens,Greece

出　　处：《Insect Science》2023年第5期1378-1392,共15页昆虫科学（英文版）

基　　金：supported by Guangzhou Science and Technology Plan(202201010369);Natural Science Foundation of Guangdong Basic and Applied Basic Research Fund(2022A1515012657);National Natural Science Foundation of China(31872426);South China Agricultural University high-level talent launch project;and Guangdong Provincial Promotion Project on Preservation and Utilization of Local Breed of Livestock and Poultry(No.2018-143).

摘　　要：The Piwi-interacting RNA(piRNA)pathway has been shown to be involved in the antiviral defense against RNA viruses,especially in mosquitoes,but its universality has been questioned.Here,we used the Bombyx mori nucleopolyhedrovirus(BmNPV)-infected silkworm as a model to explore the effects of the key factors of piRNA pathway,BmAgo3 and Siwi,on replication of a large DNA virus(belonging to the family of Baculoviridae).We demonstrated that BmAgo3 and Siwi could promote the replication of BmNPV through both overexpression and knockdown experiments in BmN cell lines and silkworm larvae.In addition,we also studied the effect of PIWI-class genes on Autographa californica nucleopolyhedrovirus(AcMNPV)replication in the Spodoptera frugiperda cell line Sf9.By knocking down the expression of PIWI-class genes in Sf9,we found that Piwi-like-1 and Piwi-like-2-3 could inhibit AcMNPV replication,while Piwi-like-4-5 promoted virus replication.Our study provides compelling evidence that the piRNA pathway affects host infection by exogenous viruses in Lepidoptera.Also,our results reflect the diversity of the roles of PIWI-class genes in virus infection of the host across species.This study is the first to explore the interaction of PIWI-class proteins with DNA viruses,providing new insights into the functional roles of the piRNA pathway.

关 键 词：BmAgo3 Bombyx mori NUCLEOPOLYHEDROVIRUS Piwi-interacting RNA Siwi 

分 类 号：Q96[生物学—昆虫学]