LncRNA MRPL39通过海绵吸附miR-130靶向PTEN调控人肺癌细胞的迁移和侵袭  

Mechanism of LncRNA MRPL39 on the migration and invasion of human non-small cell lung cancer cells by sponge adsorption miR-130 regulating PTEN

作　　者：范庆浩[1] 鲍献荣 赵涵[1] FAN Qinghao;BAO Xianrong;ZHAO Han(Department of Cardiothoracic Surgery,Jinhua People’s Hospital,Jinhua 321000,China)

机构地区：[1]金华市人民医院心胸外科,浙江金华321000

出　　处：《健康研究》2023年第5期545-549,共5页Health Research

基　　金：2021年度金华市重点科学技术研究计划项目(2021-3-113)。

摘　　要：目的探究LncRNA MRPL39对人非小细胞肺癌(non-small cell lung cancer,NSCLC)迁移和侵袭的影响及机制。方法采用qPCR法检测肺癌及癌旁组织中MRPL39和miR-130的表达;构建低表达MRPL39的肺腺癌A549细胞株,CCK-8法、Transwell小室实验检测细胞增殖、迁移和侵袭,双荧光素酶报告基因验证MRPL39与miR-130靶向关系,并评价低表达MRPL39对裸鼠成瘤的影响。结果与癌旁组织比较,肺癌组织中MRPL39升高,miR-130降低;与sh-NC组比较,sh-MRPL39组细胞中MRPL39、细胞存活率、N钙黏素和波形蛋白表达、移植瘤体积及重量明显降低,迁移细胞数和侵袭细胞数减少,E钙黏素表达升高;差异均有统计学意义(P<0.05)。双荧光素酶报告基因检测显示,MRPL39与miR-130存在靶向关系;干扰miR-130表达可部分逆转沉默MRPL39对细胞增殖、迁移和侵袭的影响,及上皮间质转化转移和第10号染色体丢失的磷酸酶基因(PTEN)表达的抑制作用。结论敲低LncRNA MRPL39可抑制肺腺癌细胞增殖、迁移和侵袭能力,可能与海绵吸附miR-130调控PTEN表达有关。Objective To explore the effects and mechanism of LncRNA MRPL39 on the migration and invasion of human non-small cell lung cancer(NSCLC)cells.Methods The expressions of MRPL39 and miR-130 in lung cancer tissues and para-carcinomatissues were detected by qPCR.The lung adenocarcinoma A549 cell lines with low-expression MRPL39 were constructed.The cells proliferation,migration and invasion were detected by CCK-8 and Transwell chamber assay.The targeted relationship between MRPL39 and miR-130 was verified by dual-luciferase reporter gene assay.Theeffects of low-expression MRPL39 on nude mice tumorigenicitywere evaluated.Results Compared with para-carcinoma tissues,MRPL39 was increased,while miR-130 was decreased in lung cancer tissues(P<0.05).Compared with sh-NC group,MRPL39,cells survival rate,expressions of N-cadherin and Vimentin,volume and mass of transplanted tumors were significantly reduced,number of migration and invasion cellswas significantly decreased,and expression of E-cadherin was increased in sh-MRPL39 group(P<0.05).Dual-luciferase reporter gene assay showed that there was targeted relationship between MRPL39 and miR-130.Interfering with miR-130 expression could partially reverse the effects of silencing MRPL39 on proliferation,migration and invasion of cells,as well as the inhibition effect on epithelial-mesenchymal transition and expression of phosphatase and tensin homology deleted on chromosome ten(PTEN).Conclusions Knockingdown LncRNA MRPL39 can inhibit proliferation,migration and invasion of lung adenocarcinoma cells,which may be related to sponge adsorption miR-130 regulating PTEN.

关 键 词：非小细胞肺癌 长链非编码RNA MRPL39 miR-130 侵袭 

分 类 号：R734.2[医药卫生—肿瘤]