土贝母皂苷甲对乳腺癌细胞MCF-7凋亡的影响  

Effect of Tubeimoside I on apoptosis of breast cancer cell MCF-7

作　　者：宋铁兵 李康乐 马强 赵颖林 赵军 谢娟 SONG Tiebing;LI Kangle;MA Qiang;ZHAO Yinglin;ZHAO Jun;XIE Juan(Xi’an Hospital of Traditional Chinese Medicine,Xi’an 710021,China)

机构地区：[1]西安市中医医院,陕西西安710021 [2]西安大兴医院渭水园院区,陕西西安710018

出　　处：《陕西中医》2023年第11期1514-1517,共4页Shaanxi Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81873311)。

摘　　要：目的:探讨中药土贝母主要成分土贝母皂苷甲(TBMSⅠ)通过激活Egr1/p21信号通路对乳腺癌细胞MCF-7凋亡的影响。方法:体外培养MCF-7细胞,并把细胞分为空白组、TBMSⅠ高剂量组(20μg/ml)、TBMSⅠ中剂量组(15μg/ml)、TBMSⅠ低剂量组(10μg/ml),使用对应浓度的TBMSⅠ干预MCF-7细胞。干预24、48、72 h后,使用CCK-8法检测细胞增殖情况。干预MCF-7细胞48 h后,采用流式细胞仪检测各组细胞凋亡、细胞周期分布情况。采用Western blotting、qRT-PCR检测各组细胞Egr1、p21蛋白及mRNA表达。结果:干预24、48、72 h后,TBMSⅠ高剂量组、TBMSⅠ中剂量组的MCF-7细胞得到抑制,且抑制率呈时间与剂量依赖性。干预48 h后,TBMSⅠ高剂量组、TBMSⅠ中剂量组、TBMSⅠ低剂量组MCF-7细胞的凋亡率高于空白组,差异有统计学意义(均P<0.05)。TBMSⅠ高剂量组、TBMSⅠ中剂量组、TBMSⅠ低剂量组G0/G1期细胞占比低于空白组,G2/M期细胞高于空白组,差异有统计学意义(均P<0.05)。TBMSⅠ高剂量组、TBMSⅠ中剂量组、TBMSⅠ低剂量组Egr1及p21蛋白、mRNA表达均高于空白组,差异有统计学意义(均P<0.05)。结论:TBMSⅠ能够激活乳腺癌细胞MCF-7中Egr1/p21信号通路,诱导Egr1、p21表达,引起MCF-7细胞G2/M期周期阻滞,诱导MCF-7细胞凋亡。Objective:To investigate effect of main ingredient of Chinese medicine paniculate bolbostemma Tubeimoside I(TBMSⅠ)on apoptosis of breast cancer cell line MCF-7 by activating Egr1/p21 signaling pathway.Methods:MCF-7 cells were cultured in vitro and divided into blank group,TBMSⅠhigh-dose group(20μg/ml),TBMSⅠmedium-dose group(15μg/ml),TBMSⅠlow-dose group(10μg/ml).MCF-7 cells were treated with the corresponding concentration of TBMSⅠ.After 24,48 and 72 h of intervention,CCK-8 method was used to detect cell proliferation.After intervention of MCF-7 cells for 48 h,the cell apoptosis and cell cycle distribution in each group were detected by flow cytometry.Western blotting and qRT-PCR were used to detect the protein and mRNA expressions of Egr1 and p21 in each group.Results:After 24,48 and 72 h of intervention,MCF-7 cells in TBMSⅠhigh dose group and TBMSⅠmedium dose group were inhibited,and the inhibition rate was time-dependent and dose-dependent.After 48 h of intervention,the apoptosis rate of MCF-7 cells in TBMSⅠhigh-dose group,TBMSⅠmedium-dose group were higher than that in the blank group,difference statistically significant(all P<0.05).The proportion of G 0/G 1 phase cells in TBMSⅠhigh-dose group,TBMSⅠmedium-dose group and TBMSⅠlow-dose group were lower than those in the blank group,and G 2/M phase cells higher than that in the blank group,difference statistically significant(all P<0.05).Expression of Egr1 and p21 protein and mRNA in TBMSⅠhigh-dose group,TBMSⅠmedium-dose group and TBMSⅠlow-dose group higher than those in the blank group,difference statistically significant(all P<0.05).Conclusion:Tubeimoside I can activate Egr1/p21 signaling pathway in MCF-7 cells,increase the expression of Egr1 and p21,cause G 2/M cell cycle arrest,inducing apoptosis of MCF-7 cells.

关 键 词：乳腺癌 MCF-7细胞 土贝母皂苷甲 早期生长反应因子1 细胞凋亡 

分 类 号：R737.9[医药卫生—肿瘤]