干扰SATB1抑制宫颈癌增殖和转移能力及增强宫颈癌放射敏感性的作用研究  

Effect of interfering SATB1 on inhibiting cervical cancer proliferation and metastasis ability and enhancing radiosensitivity

作　　者：乔娟 杨昊 牛丽红 杨璐 马敬 肖慧英[3] QIAO Juan;YANG Hao;NIU Lihong;YANG Lu;MA Jing;XIAO Huiying(Department of Gynecology and Oncolgy,Peking University Oncology Hospital Inner Mongolia Hospital/Affiliated Oncology Hospital of Inner Mongolia Medical University,Hohhot,Inner Mongolia 010020,China;Department of Radiotherapy,Peking University Oncology Hospital Inner Mongolia Hospital/Affiliated Oncology Hospital of Inner Mongolia Medical University,Hohhot,Inner Mongolia 010020,China;Department of Obstetrics and Gynecology,Hohhot First Hospital,Hohhot,Inner Mongolia 010030,China)

机构地区：[1]北京大学肿瘤医院内蒙古医院/内蒙古医科大学附属肿瘤医院妇瘤科,内蒙古呼和浩特010020 [2]北京大学肿瘤医院内蒙古医院/内蒙古医科大学附属肿瘤医院放疗科,内蒙古呼和浩特010020 [3]内蒙古自治区呼和浩特市第一医院妇产科,内蒙古呼和浩特010030

出　　处：《国际检验医学杂志》2023年第21期2581-2586,共6页International Journal of Laboratory Medicine

基　　金：内蒙古自治区科技计划项目(202002135)。

摘　　要：目的研究核基质结合区结合蛋白1(SATB1)在宫颈癌组织中的表达水平及临床意义,并探讨SATB1对宫颈癌细胞增殖、转移能力和宫颈癌放疗敏感性的作用和发挥作用的分子机制。方法采用免疫组化检测SATB1在宫颈癌组织和非癌组织中的表达,并分析宫颈癌患者中SATB1的表达水平与临床病理参数的关系及对宫颈癌患者预后的影响。常规培养宫颈癌肠转移细胞(CaSki),随机分为si-NC组和si-SATB1组,分别转染NC siRNA和SATB1 siRNA,采用western blotting检测各组细胞中SATB1的表达水平,CCK8实验检测各组细胞增殖能力,Transwell实验检测各组细胞转移能力;采用不同剂量放射线处理si-NC组和si-SATB1组细胞,CCK8实验检测si-NC组和si-SATB1组细胞放疗敏感性;Western blotting检测si-NC组和si-SATB1组细胞中Wnt/β-catenin信号通路关键蛋白β-catenin及其下游蛋白细胞周期蛋白1(cyclinD1)、cMYC和基质金属蛋白酶2(MMP-2)表达水平的影响。结果与非癌组织相比,SATB1在宫颈癌组织中的表达水平显著上调(P<0.05),SATB1高表达与宫颈癌肿瘤大小、FIGO分期和淋巴结转移显著相关,且高表达SATB1的宫颈癌患者预后较差。与si-NC组相比,si-SATB1组宫颈癌细胞中SATB1蛋白的表达水平显著降低(P<0.05),si-SATB1组宫颈癌细胞增殖和转移能力显著降低(P<0.05),si-SATB1组宫颈癌细胞放疗敏感性增加(P<0.05)。si-SATB1组宫颈癌细胞中Wnt/β-catenin信号通路关键蛋白β-catenin及其下游蛋白cyclinD1、cMYC和MMP-2表达均降低(P<0.05)。结论SATB1可能通过调控Wnt/β-catenin信号通路促进宫颈癌增殖和转移能力及增强宫颈癌放射敏感性。Objective To study the expression level of special AT-rich binding protein 1(SATB1)in cervical cancer and its clinical significance,and to explore the role and molecular mechanism of SATB1 in the proliferation,metastasis and radiosensitivity of cervical cancer cells.Methods Immunohistochemistry was used to detect the expression of SATB1 in cervical cancer tissues and non-cancer tissues,and the relationship between SATB1 expression and clinicopathological parameters,the prognosis of cervical cancer patients were analyzed.The cervical cancer cells(CaSki)were routinely cultured and randomly divided into si-NC group and si-SATB1 group.They were transfected with NC siRNA and SATB1 siRNA,respectively.The expression level of SATB1 in each group was detected by Western blotting,the proliferation ability of each group was detected by CCK8 test,and the metastatic ability of each group was detected by Transwell test.The cells in the si-NC group and the si-SATB1 group were treated with different doses of radiation,and the radiosensitivity of si-NC group and the si-SATB1 group cells was detected by CCK8 test;Wnt/β-Catenin signal pathway key protein expression level ofβ-catenin and its downstream proteins cellcycle protiens cyclinD1,cMYC and matrix metalloproteinase 2(MMP-2)was detected by Western blotting test.Results Compared with the non-cancer tissues,the expression level of SATB1 in cervical cancer tissues was significantly upregulated,and the difference was statistically significant(P<0.05).Compared with the si-NC group,the expression level of SATB1 protein in cervical cancer cells in the si-SATB1 group was decreased,and the difference was statistically significant(P<0.05),the proliferation and metastasis of cervical cancer cells in the si-SATB1 group was decreased significantly,and the difference was statistically significant(P<0.05).The radiotherapy sensitivity of cervical cancer cells in the si-SATB1 group was increased,and the difference was statistically significant(P<0.05).The Wnt/β-Catenin signal pathway key pr

关 键 词：宫颈癌 核基质结合区结合蛋白1 增殖 转移 放疗敏感性 

分 类 号：R737.33[医药卫生—肿瘤]