出 处:《国际检验医学杂志》2023年第21期2592-2597,共6页International Journal of Laboratory Medicine
基 金:山西省卫生健康委员会2021年度“四个一批”科技兴医创新计划项目(2021016)。
摘 要:目的探索自噬蛋白Beclin-1和N-myc下游调控基因2(NDRG2)在食管鳞状细胞癌(ESCC)中的表达,并探讨两种蛋白作为ESCC预后分子标志物的临床价值。方法采用免疫组织化学染色检测Beclin-1与NDRG2在121例ESCC组织中的表达,通过Pearson相关分析两者表达与临床病理参数的关系。根据Beclin-1与NDRG2的表达情况将所有患者分为2组,高、低表达组的生存概率通过Kaplan-Meier曲线计算,Log-rank检验用于生存分析的比较。采用Cox比例风险模型进行单因素和多因素分析,确定ESCC患者的独立预后因子。结果免疫组织化学染色分析显示Beclin-1蛋白在121例ESCC及癌旁组织中的阳性表达率分别为40.5%(49/121)和59.5%(68/121),差异有统计学意义(χ^(2)=5.973,P=0.015)。NDRG2蛋白在ESCC组织中的阳性表达率亦明显低于癌旁组织,差异有统计学意义(46.3%vs.61.2%;χ^(2)=5.385,P=0.020)。Beclin-1低表达者有更为频繁的淋巴结转移(χ^(2)=6.158,P=0.013)和更晚的TNM分期(χ^(2)=4.538,P=0.033),而NDRG2低表达与更晚的T分期(χ^(2)=6.607,P=0.010)和TNM分期(χ^(2)=5.744,P=0.017)相关。Kaplan-Meier曲线显示,Beclin-1低表达与高表达患者的3年总体生存(OS)率分别为44.3%和60.9%,差异有统计学意义(χ^(2)=5.696,P=0.017)。NDRG2低表达者的预后亦明显低于NDRG2高表达者,3年OS率分别为43.9%和59.2%(χ^(2)=9.004,P=0.003)。单、多因素Cox比例风险模型分析表明,Beclin-1低表达(HR=1.727,95%CI:1.017~2.934,P=0.043)、NDRG2低表达(HR=1.671,95%CI:1.023~2.730,P=0.040)与TNMⅡ~Ⅲ期(HR=3.823,95%CI:2.133~6.852,P<0.001)是ESCC患者预后不良的独立预测因素。结论Beclin-1和NDRG2表达在ESCC中明显下调,且与患者不良预后相关,其可能是预测ESCC患者生存的预后标志物。Objective To explore the expression of autophagy protein Beclin-1 and N-myc downstream regulatory gene 2(NDRG2)in esophageal squamous cell carcinoma(ESCC),and to explore the clinical value of these two proteins as prognostic molecular markers for ESCC.Methods Immunohistochemical staining was used to detect the expression of Beclin-1 and NDRG2 in 121 ESCC tissues,the relationship between their expression and clinical pathological parameters through Paarson correlation analysis.Test and analyze the relationship between their expression and clinical pathological parameters.All patients were divided into two groups based on the expression of Beclin-1 and NDRG2.The survival probabilities of the high and low expression groups were calculated using Kaplan-Meier curves,and the Log-rank test was used for comparison in survival analysis.Cox proportional hazards model was used for univariate and multivariate analysis analysis to determine the independent prognostic factors of ESCC patients.Results The results of immunohistochemical staining indicated that the positive rates of Beclin-1 expression in ESCC tissues and adjacent tissues were 40.5%(49/121)and 59.5%(68/121),with significant difference(χ^(2)=5.973,P=0.015).Also,the positive rate of NDRG2 expression in ESCC tissues was significantly lower than that in adjacent tissues the difference was statistically significant(46.3%vs.61.2%;χ^(2)=5.385,P=0.020).Lymph node metastasis(χ^(2)=6.158,P=0.013)was more frequent and TNM stage(χ^(2)=4.538,P=0.033)was more advanced in ESCC patients with low Beclin-1 expression than those with high Beclin-1 expression,and low expression of NDRG2 was associated with advanced T stage(χ^(2)=6.607,P=0.010)and TNM stage(χ^(2)=5.744,P=0.017).Kaplan-Meier curves showed that the 3-year overall survival(OS)rates of patients with low and high expression of Beclin-1 were 44.3%and 60.9%,respectively(χ^(2)=5.696,P=0.017).In addition,the 3-year OS rates of patients with low and high expression of NDRG2 were 43.9%and 59.2%,respectively(χ^(2)=9.004,
关 键 词:食管鳞状细胞癌 BECLIN-1 N-myc下游调控基因2 预后 分子标志物
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