Simultaneous Determination of 14 β-Receptor Agonists Residues in Mutton by High Performance Liquid Chromatography-Tandem Mass Spectrometry (HPLC-MS/MS)  

作　　者：Zhe MENG Jianhua WANG Bo LIU Yuhang GUO Haoshuang DONG Pingyang SHAN Dawei WANG Yajuan SONG 

机构地区：[1]Tangshan Food and Drug Comprehensive Testing Center,Tangshan 063000,China

出　　处：《Agricultural Biotechnology》2023年第5期55-58,共4页农业生物技术（英文版）

基　　金：Supported by The Fourth Batch of High-end Talent Project in Hebei Province;Tangshan Science and Technology Entrepreneurship and Innovation Leading Talent Project(21130243A).

摘　　要：[Objectives]A high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method was established for the determination of 14β-receptor agonist residues in mutton.[Methods]Samples were hydrolyzed byβ-glucuronidase and extracted with 5%acetic acid-acetonitrile(1:99,V/V)solution.An Eclipse plus C 18 column was used for separation,and the MRM mode was used for qualitative analysis,and the external standard method was used for quantitative analysis of matrix standard solutions.[Results]Under the optimal conditions,the retention time of the 14 kinds ofβ-receptor agonists ranged from 1.0 to 9.5 min.When the mass concentration was in the range of 0.05-0.50μg/ml,the linear relationship ofβ-receptor agonists was good,with correlation coefficients(r)≥0.9992.The detection limits of the method were in the range of 0.04-0.87μg/kg,and the quantitative limits were in the range of 0.35-1.86μg/kg.The average recovery values were in the range of 82.8%-108.9%,with RSDs(n=6)in the range of 1.9%-6.7%.[Conclusions]The method is simple,sensitive,reproducible,accurate,and can be used for simultaneous determination of the 14 kinds ofβ-receptor agonist residues in mutton.

关 键 词：MUTTON High performance liquid chromatography-tandem mass spectrometry β-receptor agonist RESIDUE 

分 类 号：S859.84[农业科学—临床兽医学]