盐酸药根碱对宫颈癌细胞的抑制作用  

Inhibitory Effect of Jatrorrhizine Hydrochloride on Cervical Cancer Cells

作　　者：赵冰洁 曾凤娇 魏瑶璐 董兰 贾爱亭 蔡敬 宁志丰[2] ZHAO Bing-jie;ZENG Feng-jiao;NING Zhi-feng(School of Pharmacy,Xianning Medical College,Hubei University of Science and Technology,Xianning Hubei 437100,China;不详)

机构地区：[1]湖北科技学院医学部药学院,湖北咸宁437100 [2]湖北科技学院医学部基础医学院

出　　处：《湖北科技学院学报（医学版）》2023年第5期385-389,共5页Journal of Hubei University of Science and Technology(Medical Sciences)

基　　金：湖北科技学院五官专项(202002);2022年湖北省重点研发计划(2022BCE011);湖北科技学院大学生创新创业项目(S202110927030)。

摘　　要：目的探讨盐酸药根碱(Jatrorrhizine hydrochloride,JH)在细胞水平上对宫颈癌细胞增殖能力、平板克隆、细胞伤口愈合、侵袭、迁移、细胞凋亡和坏死的影响及作用机制研究。方法通过MTT、平板克隆实验研究不同浓度的盐酸药根碱48h后对宫颈癌Hela细胞培养过程中的增殖及克隆形成的影响。为获得更准确的结果,通过细胞伤口愈合实验和Transwell细胞迁移实验来检测盐酸药根碱对Hela细胞横向和纵向迁移能力的影响,并采用Transwell细胞侵袭实验来检测其对Hela细胞侵袭能力的影响。使用YO-PRO-1/PI检测试剂盒进行荧光染色,用来检测细胞凋亡与坏死情况。通过Western blot检测不同浓度的盐酸药根碱对Hela细胞中坏死与凋亡相关蛋白表达水平的影响。结果MTT、平板克隆实验结果显示盐酸药根碱抑制宫颈癌Hela细胞增殖,呈现时间和剂量依赖;细胞伤口愈合实验和迁移、侵袭实验显示盐酸药根碱能够以剂量依赖性的方式抑制Hela细胞的横向及纵向迁移以及侵袭能力。结论体外细胞实验表明,盐酸药根碱可抑制Hela细胞增殖、侵袭、迁移能力,有望作为抗癌潜在药物在临床上进一步研究。Objective To investigate the effect of Jatrorrhizine hydrochloride(JH)on the proliferation ability,plate cloning,cell wound healing,invasion,migration,apoptosis and necrosis staining of cervical cancer cells and the mechanisms at the cellular level.Methods The effects of different concentrations of JH on the proliferation and clone formation of cervical cancer Hela cells after 48 h of cultivation were studied by the MTT and plate cloning methods.To obtain more accurate results,the effects of JH on the lateral and longitudinal migration ability of Hela cells were examined by the cell wound healing assay and Transwell cell migration assay,and meanwhile the invasion ability of Hela cells were detected by the Transwell cell invasion assay.Fluorescent staining was performed to detect the apoptosis and necrosis of cells by using YOPRO1/PI assay kit.The effects of different concentrations of JH on the expression levels of necrosis and apoptosis related proteins in Hela cells were examined by the Western blot.Results The results of MTT and plate cloning assays showed that JH inhibited the proliferation of cervical cancer Hela cells in a time and dosedependent manner.The cell wound healing assay and migration and invasion assays showed that JH could inhibit the lateral and longitudinal migration and invasion ability of Hela cells in a dosedependent manner.Conclusion In vitro cellular assays showed that JH can inhibit the proliferation,invasion and migration ability of Hela cells,and is expected to be further investigated as a potential anticancer drug in the clinical practice.

关 键 词：宫颈癌 盐酸药根碱 HELA 凋亡 

分 类 号：R737.33[医药卫生—肿瘤]