LASP1基因对人结直肠癌LOVO细胞增殖、迁移和凋亡的影响及其机制  

Effects and Mechanism of LASP1 on Proliferation,Migration,and Apoptosis of Human Colorectal Cancer LOVO Cells

作　　者：徐益平[1] 尚韬 XU Yiping;SHANG Tao(Department of Pathology,Hangzhou Cancer Hospital,Hangzhou 310005,China;Department of Anorectal Diseases,The First Af filiated Hospital of Zhejiang Chinese Medicine University(Zhejiang Provincial Hospital of Chinese Medicine),Hangzhou 310018,China)

机构地区：[1]杭州市肿瘤医院病理科,杭州310005 [2]浙江中医药大学附属第一医院(浙江省中医院)肛肠科,杭州310018

出　　处：《肿瘤防治研究》2023年第10期955-959,共5页Cancer Research on Prevention and Treatment

基　　金：浙江省医药卫生科技计划项目(2021KY823)。

摘　　要：目的探讨LASP1基因表达对人结肠癌(LOVO)细胞增殖、迁移以及侵袭能力的影响及其作用机制。方法分别构建LASP1过表达质粒和LASP1干扰质粒转染LOVO细胞,qRT-PCR验证LASP1mRNA转染结果。MTT法、Tunel染色分别检测细胞增殖活性和细胞的凋亡情况,细胞划痕和Transwell实验检测细胞迁移和侵袭能力。Westernblot测定细胞LASP1、p-FAK/FAK、p-AKT/AKT蛋白表达。结果质粒转染成功。LASP1过表达的LOVO细胞的增殖、迁移和侵袭能力增加,细胞凋亡率降低,细胞中LASP1、p-FAK/FAK、p-AKT/AKT蛋白表达升高(P<0.01)。而敲减LOVO细胞中LASP1的表达后,细胞增殖、迁移和侵袭能力减弱,细胞凋亡率增加,细胞中LASP1、p-FAK/FAK、p-AKT/AKT蛋白表达降低(P<0.01)。结论LASP1可正向调控FAK/AKT信号通路,促进LOVO细胞的增殖、迁移以及侵袭能力。Objective To explore the effects and mechanism of LASP1 gene expression on the proliferation,migration,and invasion of human colorectal cancer(LOVO)cells.Methods LASP1 overexpression plasmids and LASP1 interference plasmids were constructed and transfected to LOVO cells.qRT-PCR was used to detect LASP1 mRNA expression and validate the transfection.MTT method and Tunel staining were used to detect cell proliferation and apoptosis,respectively,and scratch test and Transwell test were employed to determine the migration and invasion abilities of cells.Western blot was applied to analyze the expression of LASP1,p-FAK/FAK,and p-AKT/AKT protein in cells.Results The plasmids were successfully transfected.LASP1 overexpression increased the proliferation,migration,and invasion of LOVO cells,decreased the apoptosis,and increased LASP1,p-FAK/FAK,p-AKT/AKT protein expression(P<0.01).LASP1 knockdown reduced the proliferation,migration,and invasion of LOVO cells,increased the apoptosis,and decreased LASP1,p-FAK/FAK,and p-AKT/AKT protein expression(P<0.01).Conclusion LASP1 positively regulates the FAK/AKT signaling pathway to promote the proliferation,migration,and invasion of LOVO cells.

关 键 词：结直肠癌 LOVO细胞 LASP1基因 转移 侵袭 FAK/AKT信号通路 

分 类 号：R735.3[医药卫生—肿瘤]