EPA对P.gingivalis LPS诱导人牙周膜成纤维细胞炎症反应的影响  

Effect of EPA on inflammatory response of human periodontal ligament fibroblasts induced by P.gingivalis LPS

作　　者：周子超[1] 束蓉 吴轶群[1] ZHOU Zi-chao;SHU Rong;WU Yi-qun(Department of 2nd Dental Center,Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,College of Stomatology,Shanghai Jiao Tong University,National Center for Stomatology,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology,Shanghai Research Institute of Stomatology.Shanghai 200011,China;Department of Periodontology,Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,College of Stomatology,Shanghai Jiao Tong University,National Center for Stomatology,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology,Shanghai Research Institute of Stomatology.Shanghai 200011,China)

机构地区：[1]上海交通大学医学院附属第九人民医院口腔第二门诊部,上海交通大学口腔医学院,国家口腔医学研究中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海200011 [2]海交通大学口腔医学院,国家口腔医学研究中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所牙周病科,上海200011

出　　处：《上海口腔医学》2023年第5期475-479,共5页Shanghai Journal of Stomatology

摘　　要：目的:分析二十碳五烯酸(eicosapntemacnioc acid,EPA)对牙龈卟啉单胞菌(Porphyromonas gingivalis,P.gingivalis)脂多糖(lipopolysaccharides,LPS)诱导人牙周膜成纤维细胞(human periodontal ligament cells,hPDLCs)分泌炎症因子的作用。方法:利用组织块法培养hPDLCs,MTT法检测不同浓度EPA对hPDLCs细胞活性的影响。根据MTT结果选择合适的EPA浓度,分别采用实时定量PCR和ELISA法检测EPA对P.gingivalis LPS诱导hPDLCs分泌白细胞介素6(Interleukin-6,IL-6)、IL-8和IL-1β能力的影响,采用SPSS 10.0软件包对数据进行统计学分析。结果:25~100μmol/L EPA对hPDLCs细胞活性无影响,但可呈剂量依赖性下调P.gingivalis LPS诱导hPDLCs分泌IL-6、IL-8和IL-1β的能力。结论:EPA有望在不影响细胞活性的情况下,抑制P.gingivalis LPS诱导hPDLCs分泌炎症因子的能力,EPA可能成为牙周炎抗炎治疗的潜在靶点。PURPOSE:To observe the effect of eicosapentaenoc acid(EPA)on the expression of inflammatory factors in human periodontal ligament fibroblasts(hPDLCs)induced by using lipopolysaccharides(LPS)of Porphyromonas gingivalis(P.gingivalis).METHODS:hPDLCs were cultured by using tissue block method,and the effects of different concentrations of EPA on the activity of hPDLCs cells were observed by MTT method.According to MTT results,the appropriate concentration of EPA was selected,and the expression of interleukin-6(IL-6),IL-8 and IL-1βin hPDLCs induced by P.gingivalis LPS was detected by real time PCR and ELISA.The data were evaluated by SPSS 10.0 software package.RESULTS:25-100μmol/L EPA had no effect on the activity of hPDLCs cells,but could inhibit the expression of IL-6,IL-8 and IL-1βinduced by P.gingivalis LPS in a dose-dependent manner.CONCLUSIONS:EPA can inhibit the expression of inflammatory factors induced by P.gingivalis LPS without affecting cell activity,indicating that EPA has the possibility of anti-inflammatory treatment of periodontitis.

关 键 词：二十碳五烯酸 脂多糖 人牙周膜成纤维细胞 炎症因子 

分 类 号：R781.4[医药卫生—口腔医学]