SSTF上调miR-495表达减缓高糖诱导的视网膜神经节细胞氧化损伤和凋亡  

SSTF up-regulates the expression of miR-495 and attenuates the oxidative damage and apoptosis of retinal ganglion cells induced by high glucose

作　　者：白玫[1] 苗得雨 李雅丽[1] 李亚坤[1] 吕建东 郭向东[1] 吴忧 BAI Mei;MIAO Deyu;LI Yali;LI Yakun;L Jiandong;GUO Xiangdong;WU You(Department of Ophthalmology,the Second Affiliated Hospital of Hebei North University,Zhangjiakou 075100,Hebei,China;Department of Pharmacy,Hebei Xuangang Hospital,Zhangjiakou 075100,Hebei,China;Department of Traditional Chinese Medicine,the Second Affiliated Hospital of Hebei North University,Zhangjiakou 075100,Hebei,China;Department of Ophthalmology,The Second Hospital of Qinhuangdao City,Qinhuangdao 066600,Hebei,China)

机构地区：[1]河北北方学院附属第二医院眼科,张家口075100 [2]河北宣钢医院药剂科,张家口075100 [3]河北北方学院附属第二医院中医科,张家口075100 [4]河北省秦皇岛市第二医院眼科,秦皇岛066600

出　　处：《医学研究与战创伤救治》2023年第6期573-578,共6页Journal of Medical Research & Combat Trauma Care

基　　金：河北省中医药管理局科研计划项目(2021187)。

摘　　要：目的探讨黄岑茎叶总黄酮(SSTF)对高糖诱导的视网膜神经节细胞(RGC-5)损伤的保护机制。方法根据处理方法不同将RGC-5细胞分成高糖(HG)组、对照(Con)组、HG+SSTF-低剂量(L)组、HG+SSTF-中剂量(M)组、HG+SSTF-高剂量(H)组、HG+miR-NC组、HG+miR-495组、HG+SSTF+anti-miR-NC组、HG+SSTF+anti-miR-495组,每组设置9复孔。检测MDA含量以及CAT、SOD活性。流式细胞术检测细胞凋亡。实时荧光定量PCR(qRT-PCR)检测miR-495表达。结果HG组RGC-5细胞凋亡率、MDA含量高于Con组(P<0.05),CAT、SOD的活性、miR-495表达低于Con组(P<0.05)。HG+SSTF-L组、HG+SSTF-M组、HG+SSTF-H组RGC-5细胞凋亡率、MDA含量低于HG组(P<0.05),CAT和SOD的活性、miR-495表达高于HG组(P<0.05)。HG+miR-495组RGC-5细胞凋亡率[(14.96±1.26)%]、MDA含量[(4.62±0.44)μmol/g]分别低于HG+miR-NC组[(33.28±2.25)%、(9.73±0.81)μmol/g],差异有统计学意义(P<0.05),CAT、SOD的活性、miR-495表达水平高于HG+miR-NC组(P<0.05)。HG+SSTF+anti-miR-495组RGC-5细胞凋亡率[(22.21±2.18%)]、MDA含量[(8.07±0.75)μmol/g]高于HG+SSTF+anti-miR-NC组[(9.19±0.84)%、(3.59±0.32)μmol/g],差异有统计学意义(P<0.05),CAT和SOD的活性、miR-495表达水平低于HG+SSTF+anti-miR-NC组(P<0.05)。结论SSTF通过上调miR-495表达减缓高糖诱导的视网膜神经节细胞氧化损伤和凋亡。Objective To investigate the protective mechanism of total flavonoids from stems and leaves of Scutellaria baicalensis(SSTF)on high glucose-induced retinal ganglion cell(RGC-5)injury.Methods According to different treatment methods,RGC-5 cells were divided into high glucose(HG)group,control(Con)group,HG+SSTF low-dose(L)group,HG+SSTF medium-dose(M)group,HG+SSTF high-dose(H)group,HG+miR-NC group,HG+miR-495 group,HG+SSTF+anti-miR-NC group,HG+SSTF+anti-miR-495 group,and 9 multiple groups were set up in each group.The content of MDA and the activities of CAT and SOD were detected.Cell apoptosis was detected by flow cytometry.The expression of miR-495 was detected by real-time fluorescence quantitative PCR(qRT-PCR).Results The apoptosis rate and MDA content of RGC-5 cells in HG group were higher than those in Con group(P<0.05),and the activities of CAT,SOD and miR-495 expression in HG group were lower than those in Con group(P<0.05).The apoptosis rate and MDA content of RGC-5 cells in HG+SSTF(L),HG+SSTF(M)and HG+SSTF(H)groups were lower than those in HG group(P<0.05),and the activities of CAT,SOD and miR-495 expression were higher than those in HG group(P<0.05).The apoptosis rate[(14.96±1.26)%]and MDA content[(4.62±0.44)μmol/g]of RGC-5 cells in HG+miR-495 group were lower than those in HG+miR-NC group[(33.28±2.25)%and(9.73±0.81)μmol/g],respectively.The differences were statistically significant(P<0.05),and the activities of CAT,SOD and miR-495 expression levels were higher than those of HG+miR-NC group(P<0.05).The apoptosis rate of RGC-5 cells[(22.21±2.18%)]and MDA content[(8.07±0.75)μmol/g]in HG+SSTF+anti-miR-495 group were higher than those in HG+SSTF+anti-miR-NC group[(9.19±0.84)%and(3.59±0.32)μmol/g],the difference was statistically significant(P<0.05),and the activities of CAT,SOD and expression level of miR-495 were lower than those of HG+SSTF+anti-miR-NC group(P<0.05).Conclusion SSTF can inhibit the oxidative damage and apoptosis of retinal ganglion cells induced by high glucose by up-regulating the

关 键 词：视网膜神经节细胞 黄岑茎叶总黄酮 miR-495 细胞凋亡 

分 类 号：R774[医药卫生—眼科]