结直肠癌肝转移患者白细胞介素-18、促肝再生磷酸酶蛋白3表达与PI3K及AKT蛋白的关系  

Correlation among IL-18,PRL-3,PI3K and AKT in patients with colorectal cancer and liver metastasis

作　　者：罗振凌[1] 顾群浩[1] LUO Zhenling;GU Qunhao(Department of Gastrointestinal Surgery,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China)

机构地区：[1]上海中医药大学附属岳阳中西医结合医院胃肠外科,上海200437

出　　处：《医学研究与战创伤救治》2023年第6期609-614,共6页Journal of Medical Research & Combat Trauma Care

摘　　要：目的探讨结直肠癌肝转移患者癌组织中白细胞介素-18(IL-18)、促肝再生磷酸酶蛋白3(PRL-3)表达与临床病理特征及磷脂酰肌醇3-激酶(PI3K)、丝苏氨酸蛋白激酶B(AKT)蛋白水平的关系。方法选取2019年10月至2021年10月于上海中医药大学附属岳阳中西医结合医院行同期切除术的85例结直肠癌肝转移患者(肝转移组)的癌组织样本及癌旁正常组织(距肿瘤切缘4 cm),以及同期65例未发生肝转移结直肠癌患者(未肝转移组)的癌组织为研究对象。免疫组织化学S-P法检测不同组织中IL-18、PRL-3阳性表达率,Western blot检测癌组织中PI3K、p-AKT相对表达水平,分析IL-18、PRL-3与患者临床病理的关系及其与PI3K、p-AKT表达的相关性。结果与未肝转移组比较,肝转移组TNM分期IV期、低分化程度比例较高(P<0.05)。肝转移患者癌组织中IL-18、PRL-3、PI3K、p-AKT蛋白阳性表达率高于其癌旁正常组织和未肝转移患者癌组织,且未肝转移患者癌组织中IL-18、PRL-3、PI3K、p-AKT蛋白阳性表达率高于肝转移患者癌旁正常组织(P<0.05)。相关性分析示,肝转移患者癌组织中IL-18、PRL-3表达与PI3K、p-AKT均呈正相关(P<0.05);未转移癌组织中IL-18表达与PI3K、p-AKT均呈正相关(P<0.05),PRL-3表达与p-AKT均呈正相关(P<0.05),且IL-18与PRL-3呈显著正相关(P<0.05)。结论IL-18、PRL-3可能通过激活PI3K/AKT信号通路从而参与结直肠癌肝转移过程。Objective To explore the correlation of IL-18,PRL-3,PI3K and AKT in patients with colorectal cancer and liver metastasis.Methods The samples of colorectal cancertissues and normal para-cancerous tissues were prospectivelycollected from 85 patients with colorectal cancer and liver metastasiswho underwent radical operation in the hospital between October 2019 and October 2021(liver metastasis group),andthe samples of cancer tissues were also collected from 65 colorectal cancer patients without liver metastasis during the same period(non-liver metastasis group).The positive expression rates of IL-18 and PRL-3were detected by immunohistochemistry(IHC).The relative expression levels of PI3K and AKT in cancertissueswere detected by Western blot.The relationship between IL-18/PRL-3 and clinicopathology,and their correlation with PI3K and AKT were analyzed.Results Compared to the non-liver metastasis group,the liver metastasis group in TNM staging IVhad higher differentiation degree(P<0.05).The positive rates of IL-18 and PRL-3,and the expression levels of PI3K and AKT in cancer tissuesin patients with colorectal cancer and liver metastasis were higher than those in para-canceroustissues and cancer tissues in those without liver metastasis,and the expression levels in cancer tissues in patients without liver metastasis were higher than those in normal para-cancerous tissuesin patients with liver metastasis(P<0.05).For the correlation analysis,the expression levels of IL-18/PRL-3,and PI3K/AKT in the liver metastasis groupwere positive(P<0.05),and the expression levels of IL-18 and PI3K/AKT,PRL-3 and AKT,IL-18 and PRL-3 in the non-liver metastasis group were also positive(P<0.05).Conclusion IL-18 and PRL-3 may activate PI3K/AKT and play certain roles incolorectal cancer and liver metastasis.

关 键 词：结直肠癌 肝转移 白细胞介素-18 促肝再生磷酸酶蛋白3 磷脂酰肌醇3-激酶 丝苏氨酸蛋白激酶B 

分 类 号：R735.3[医药卫生—肿瘤]