多瘤病毒增强活化子3和红细胞生成素产生肝细胞受体A2在脑胶质母细胞瘤中的作用  

The role of polyomavirus enhancer activator 3 and erythropoietin-producing hepatocellular receptor A2 in glioblastoma of the brain

作　　者：麦麦提依明·托合提 张诚[1] 高峰[1] 王继超[1] 吴永刚[1] Mamatemin Tohti;Zhang Cheng;Gao Feng;Wang Jichao;Wu Yonggang(Department of Neurosurgery,People′s Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830001,China)

机构地区：[1]新疆维吾尔自治区人民医院神经外科,乌鲁木齐830001

出　　处：《中国医药》2023年第11期1646-1650,共5页China Medicine

基　　金：新疆维吾尔自治区自然科学基金(2022D01C625、2019D01C107)。

摘　　要：目的探讨多瘤病毒增强活化子3(PEA3)、红细胞生成素产生肝细胞受体A2(EPHA2)在脑胶质母细胞瘤中的作用。方法构建人脑星形胶质母细胞瘤U87细胞基因转染模型,将细胞系分为5组:空白组、PEA3干扰组、PEA3干扰空载组、EPHA2干扰组、EPHA2干扰空载组。通过细胞计数试剂盒8(CCK-8)实验检测细胞增殖率,细胞划痕实验检测细胞迁移能力,Transwell实验检测细胞侵袭能力。结果CCK-8检测细胞增殖结果显示,PEA3干扰组和EPHA2干扰组的细胞增殖率均明显低于空白组[(59.7±1.3)%、(59.5±0.7)%比(67.8±1.3)%](P<0.01或P<0.001),PEA3干扰空载组和EPHA2干扰空载组的细胞增殖率与空白组比较差异均无统计学意义(均P>0.05)。细胞划痕实验结果显示,PEA3干扰组和EPHA2干扰组的划痕愈合率低于空白组[(31.7±1.1)%、(23.0±2.6)%比(42.8±8.8)%],差异均有统计学意义(均P<0.05),PEA3干扰空载组和EPHA2干扰空载组划痕愈合率与空白组比较差异均无统计学意义(均P>0.05)。Transwell检测细胞侵袭能力结果显示,PEA3干扰组和EPHA2干扰组的侵袭细胞数明显少于空白组[(643±20)、(542±165)个比(1225±70)个](均P<0.001),而空白组与PEA3干扰空载组和EPHA2干扰空载组侵袭细胞数比较差异均无统计学意义(均P>0.05)。结论干扰PEA3基因和EPHA2基因能明显降低脑胶质母细胞瘤细胞的增殖、迁移、侵袭能力。Objective To investigate the role of polyomavirus enhancer activator 3(PEA3)and erythropoietin-producing hepatocellular receptor A2(EPHA2)in glioblastoma of the brain.Methods The gene transfection model of glioblastoma U87 cells was constructed.The cell lines were divided into five groups:blank group,PEA3 interference group,PEA3 interference empty group,EPHA2 interference group and EPHA2 interference empty group.The proliferation rate was detected by cell count kit-8(CCK-8)test,the migration ability was detected by cell scratch test,and the invasion ability was detected by Transwell test.Results CCK-8 test showed that compared with the blank group,the cell proliferation rate of PEA3 interference group and EPHA2 interference group decreased significantly[(59.7±1.3)%,(59.5±0.7)%vs(67.8±1.3)%](P<0.01 or P<0.001).There was no significant difference between the blank group and PEA3 interference empty group and EPHA2 interference empty group in cell proliferation rate(both P>0.05).Cell scratch test showed that compared with the blank group,the cell scratch healing rate of PEA3 interference group and EPHA2 interference group decreased[(31.7±1.1)%,(23.0±2.6)%vs(42.8±8.8)%](both P<0.05).There was no significant difference between the blank group and PEA3 interference empty group and EPHA2 interference empty group in scratch healing rate(both P>0.05).Transwell test results showed that compared with the blank group,the number of invasive cells in PEA3 interference group and EPHA2 interference group decreased significantly[(643±20),(542±165)cells vs(1225±70)cells](both P<0.001),and there was no significant difference between the blank group and PEA3 interference empty group and EPHA2 interference empty group(both P>0.05).Conclusion Interference with PEA3 gene and EPHA2 gene can significantly reduce the proliferation,migration and invasion of glioblastoma of the brain.

关 键 词：胶质母细胞瘤 多瘤病毒增强活化子3 红细胞生成素产生肝细胞受体A2 

分 类 号：R739.41[医药卫生—肿瘤]