干扰sorcin抑制乳腺癌细胞的侵袭转移  

Sorcin Silencing Inhibits Migration and Invasion of Breast Cancer Cells

作　　者：蔡培英 丁洁[1] 姜博 马新梅[1] 王一婷 李双静 CAI Peiying;DING Jie;JIANG Bo(Liaocheng People's Hospital,Liaocheng,252000)

机构地区：[1]山东省聊城市人民医院,252000

出　　处：《实用癌症杂志》2023年第11期1760-1763,1768,共5页The Practical Journal of Cancer

基　　金：山东省自然科学基金(编号:ZR2015HL068);山东省博士后创新基金(编号:140223)。

摘　　要：目的 研究siRNA干扰sorcin(soluble resistance-related calcium-binding protein)对乳腺癌细胞MDA-MB-231侵袭和迁移的影响。方法 利用lipofectamine将sorcin siRNA及阴性对照转染至MDA-MB-231细胞;real-time PCR和western blot方法检测sorcin的干扰效率;MTT方法检测细胞对化疗药物的敏感性;细胞划痕实验、Transwell小室法检测细胞的迁移和侵袭能力;real-time PCR和western blot方法检测E-钙黏蛋白(E-cadherin)表达。结果 sorcin siRNA干扰可显著降低MDA-MB-231细胞中sorcin表达;干扰sorcin后MDA-MB-231细胞对化疗药物敏感性提高;细胞迁移和侵袭能力显著下降;E-cadherin表达在mRNA水平和蛋白水平均显著性升高。结论 干扰sorcin后可显著性抑制MDA-MB-231细胞的侵袭迁移能力,其机制可能是与上调E-cadherin的表达有关。Objective To investigate the effect of small interfering RNA(siRNA)targeting soluble resistance-related calcium-binding protein(sorcin)on the migration and invasion of MDA-MB-231 breast cancer cells.Methods Sorcin siRNA and negative control siRNA were transfected into MDA-MB-231 cells by lipofectamine package.The expression level of sorcin was detected by real-time PCR and western blot.MTT assay was used to determine the sensitivity of cells to chemotherapeutic drugs.The migration and invasion ability of cells were examined by wound healing assay and transwell assay.E-cadherin expression level was examined by real-time PCR and western blot.Results After transfection of sorcin siRNA into MDA-MB-231 cells,the expression level of sorcin mRNA and protein was decreased significantly.sorcin silencing increased the drug sensitivity and inhibited migration and invasion of MDA-MB-231 cells.The expression level of E-cadherin mRNA and protein was increased after down-regulation of sorcin.Conclusion Silencing of sorcin could inhibit migration and invasion of MDA-MB-231 cells,which may by up-regulation of E-cadherin expression.

关 键 词：SORCIN 乳腺癌 小干扰RNA 肿瘤转移 

分 类 号：R737.9[医药卫生—肿瘤]