基于Wnt/β-catenin信号通路探讨阿司匹林联合阿托伐他汀对非小细胞肺癌细胞A549细胞增殖的影响  

Exploration on the Effects of Aspirin Combined with atorvastatin on the Proliferation of A549 Cells in Non-small Cell Lung Cancer Based on Wnt/β-catenin Signaling Pathways

作　　者：朱莹 易善永 郭云霞[2] 吕朋举 ZHU Ying;YI Shanyong;GUO Yunxia(Zhengzhou Central Hospital Affiliated to Zhengzhou University,Zhengzhou,450007)

机构地区：[1]郑州大学附属郑州中心医院,450007 [2]郑州人民医院,450007

出　　处：《实用癌症杂志》2023年第11期1764-1768,共5页The Practical Journal of Cancer

摘　　要：目的基于Wnt/β-catenin信号通路探讨阿司匹林(ASA)联合阿托伐他汀(Atorvastatin)对非小细胞肺癌(NSCLC)A549细胞增殖的影响。方法将A549细胞株分为Control组、ASA组、Atorvastatin组和ASA+Atorvastatin组。CCK-8法检测细胞存活率;ELISA检测炎症因子和氧化应激水平;Western blot检测蛋白表达。结果CCK-8结果显示,ASA(≥50μmol/L)、Atorvastatin(≥2μmol/L)和ASA联合Atorvastatin均能明显抑制A549细胞增殖,这种抑制作用随药物作用浓度增加而增加(P<0.05)。与Control组相比,ASA组、Atorvastatin组和ASA+Atorvastatin组氧化应激和炎症水平明显降低(P<0.05);与ASA组和Atorvastatin组相比,ASA+Atorvastatin组氧化应激和炎症水平明显降低(P<0.05)。与Control组相比,ASA组、Atorvastatin组和ASA+Atorvastatin组Wnt和β-catenin蛋白表达量均明显下降(P<0.05);与ASA组和Atorvastatin组相比,ASA+Atorvastatin组Wnt和β-catenin蛋白表达量明显下降(P<0.05)。结论ASA联合Atorvastatin能够抑制Wnt/β-catenin信号通路有效抑制A549细胞增殖。Objective To explore the effects of aspirin(ASA)combined with atorvastatin(Atorvastatin)on the proliferation of A549 cells in non-small cell lung cancer(NSCLC)based on Wnt/β-catenin signaling pathways.Methods A549 cells were divided into the control group,ASA group,Atorvastatin group and ASA+Atorvastatin group.The survival rate of cells was detected by CCK-8.The levels of inflammatory factors and oxidative stress were detected by ELISA.The expressions of related proteins were detected by Western blot.Results CCK-8 results showed that ASA(≥50μmol/L),Atorvastatin(≥2μmol/L)and ASA combined with Atorvastatin could significantly inhibit the proliferation of A549 cells,showing concentration dependence(P<0.05).Compared with the control group,levels of oxidative stress and inflammation were significantly decreased in the other 3 groups(P<0.05).Compared with ASA group and Atorvastatin group,levels of oxidative stress and inflammation were significantly decreased in ASA+Atorvastatin group(P<0.05).Compared with the control group,expressions of Wnt andβ-catenin were significantly decreased in the other 3 groups(P<0.05).Compared with ASA group and Atorvastatin group,expressions of Wnt andβ-catenin were significantly decreased in ASA+Atorvastatin group(P<0.05).Conclusion ASA combined with Atorvastatin can effectively inhibit the proliferation of A549 cells by inhibiting Wnt/β-catenin signaling pathways.

关 键 词：Wnt基因 Β-连环蛋白 阿司匹林 阿托伐他汀 非小细胞肺癌 细胞增殖 

分 类 号：R734.2[医药卫生—肿瘤]