宫颈癌及癌前病变中的HPV-16整合人宿主基因组的发生情况及其在宫颈癌筛查中的应用  

Study on the Integration Status and Clinical Value of HPV16 DNA in Cervical Cancer and Precancerous Lesions

作　　者：郭瑞丽[1] 朱科科[1] 赵静[1] GUO Ruili;ZHU Keke;ZHAO Jing(Jiaozuo People's Hospital,Jiaozuo,454000)

机构地区：[1]河南省焦作市人民医院,454000

出　　处：《实用癌症杂志》2023年第11期1810-1812,1816,共4页The Practical Journal of Cancer

摘　　要：目的 探讨宫颈癌及癌前病变中的HPV-16整合人宿主基因组的发生情况及其在宫颈癌筛查中的应用。方法 收集宫颈癌患者50例、 LSIL 127例、HSIL 83例,另外收集同时期行子宫全切术提取的正常宫颈上皮组织22例。采用重叠PCR检测HPV-16感染病毒整合状态。结果 正常宫颈上皮组织、 LSIL、HSIL及宫颈癌组织中,HR-HPV阳性检出率分别为13.64%、33.07%、61.45%以及94.00%,上述任意两组进行统计分析,P<0.05。正常宫颈上皮组织、 LSIL、HSIL及宫颈癌组织中,HPV-16感染率分别为4.55%、18.90%、36.14%以及66.00%,上述任意两组进行统计分析,P<0.05。正常宫颈上皮组织、 LSIL、HSIL及宫颈癌组织中,HPV-16整合率分别为0、17.32%、9.64%以及58.00%,上述任意两组进行统计分析,P<0.05。结论 HPV16整合感染与宫颈病变疾病进展有关,在HPVDNA检测的基础上联合对高危HPV整合感染状态进行检测,有利于提高宫颈癌筛查准确率并在早期预测宫颈病变的转归。Objective To investigate the occurrence of HPV-16 integrated human host genome in cervical cancer and precancerous lesions and its application in cervical cancer screening.Methods 50 cases of cervical cancer,127 cases of LSILa,83 cases of HSILA were collected,and 22 cases of normal cervical epithelial tissues were collected.The integration status of HPV-16 infection was detected by overlapping PCR.Results The positive rates of HR-HPV in normal cervical epithelium,LSIL,HSIL and cervical cancer were 13.64%,33.07%,61.45%and 94.00%,respectively.Statistical analysis was performed in any two groups(P<0.05).The infection rates of HPV-16 in normal cervical epithelial tissue,LSIL,HSIL and cervical cancer tissues were 4.55%,18.90%,36.14%and 66.00%,respectively.Statistical analysis was performed in any two groups,P<0.05.HPV-16 integration rates in normal cervical epithelia,LSIL,HSIL and cervical cancer tissues were 0,17.32%,9.64%and 58.00%,respectively.Statistical analysis was performed in any two groups,P<0.05.Conclusion HPV16 integrated infection is associated with the progression of cervical lesions.Detection of high-risk HPV integrated infection based on HPVDNA detection is beneficial to improve the accuracy of cervical cancer screening and predict the outcome of cervical lesions in the early stage.

关 键 词：宫颈癌 人乳头瘤病毒 整合状态 临床价值 

分 类 号：R737.33[医药卫生—肿瘤]