茶树新梢响应茶蚜取食的代谢物变化研究  

Metabolite changes in new shoots of Camellia sinensis(L.)O.Kuntze infested by tea aphids

作　　者：刘艳丽[1] 唐海燕[2] 万晴 颜亚敏 马林龙[1] 曹丹 金孝芳[1] Liu Yan-Li;Tang Hai-Yan;Wan Qing;Yan Ya-Min;Ma Lin-Long;Cao Dan;Jin Xiao-Fang(Institute of Fruit and Tea,Hubei Academy of Agricultural Sciences,Wuhan 430064,China;Department of Horticulture,Hubei Vocational College of Biotechnology,Wuhan 430070,China;College of Life Science and Technology,Hubei University,Wuhan 430062,China)

机构地区：[1]湖北省农业科学院果树茶叶研究所,武汉430064 [2]湖北生物科技职业学院园艺系,武汉430070 [3]湖北大学生命科学学院,武汉430062

出　　处：《植物科学学报》2023年第5期657-667,共11页Plant Science Journal

基　　金：国家重点研发计划项目(2021YFD1601103);湖北省重点研发计划项目(2021BBA241);湖北省茶叶创新团队项目(2021-620-000-001-24)。

摘　　要：茶蚜是春季茶树(Camellia sinensis(L.)O.Kuntze)的主要害虫,严重影响茶叶的产量和品质。次生代谢物质在植物防御蚜虫危害过程中具有重要作用,研究茶蚜为害下茶树新梢次生代谢物的变化有利于筛选和培育抗蚜茶树品种。本研究以茶蚜为害和未为害的茶树品种‘鄂茶一号’新梢为材料,开展了代谢组学分析和主要代谢物定量分析,检测了苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)活性和脯氨酸(Pro)含量。结果显示,‘鄂茶一号’茶蚜危害率高达35.4%,对茶蚜中等抗性。茶蚜为害后,新梢中PAL活性轻微上升,PPO活性显著提高,可溶性糖、黄酮和Pro含量显著上升,而茶多酚含量显著下降。茶蚜为害诱导了8个代谢物质显著差异变化,包括3个初生代谢物和5个次生代谢物。3个初生代谢物全部上调表达,可能为次生代谢物合成提供前体,也可能改变茶蚜生存所需的营养物质。5个次生代谢物包括1个茉莉酸类物质JA-Ile和4个类黄酮物质,JA-Ile作为防御信号物质显著上调表达,激活茶树下游防御体系,类黄酮作为防御物质直接参与茶蚜防御。研究结果表明JA-Ile、类黄酮和Pro可能是茶树防御茶蚜的主要物质。Tea aphids are a major insect pest of tea plants(Camellia sinensis(L.)O.Kuntze),resulting in both tea production loss and tea quality decline.Secondary metabolites play vital roles in plant resistance against aphid feeding.Therefore,understanding changes in metabolites before and after tea aphid infestation is essential for the screening and breeding of aphid-resistant tea varieties.In the current study,metabolomics profiling,quantitative analysis of metabolites,and determination of phenylalanine ammonia-lyase(PAL)and polyphenol oxidase(PPO)activities and proline(Pro)content were performed in new shoots of the‘Echa 1’tea variety infested with tea aphids,using non-infested plants as controls.Results showed that 35.4%of‘Echa 1’was colonized by tea aphids,suggesting that‘Echa 1’exhibited medium resistance to tea aphids.In parallel,significantly higher PPO activity,markedly higher soluble sugar,total flavonoid,and Pro contents,and significantly lower tea polyphenol content were found after aphid infestation.Eight significantly accumulated metabolites,including three primary metabolites and five secondary metabolites,were also detected.The three primary metabolites were all up-regulated in aphid-impacted shoots,which may confer precursors for secondary metabolite biosynthesis and modify the nutritional compounds utilized by tea aphids.The five secondary metabolites included jasmonate-isoleucine(JA-Ile)and four flavonoids.JA-Ile,responsible for defense-related signaling molecules,was significantly up-regulated after aphid-feeding,functioning in the activation of downstream defensive pathways,while the flavonoids served as defense compounds against the tea aphids.Taken together,these results suggest that JA-Ile,flavonoids,and Pro are important defensive compounds against tea aphids in tea plants.

关 键 词：茶树 新梢 茶蚜 代谢物质 防御 

分 类 号：Q945[生物学—植物学]