牛肉样品中产志贺毒素大肠埃希氏菌和肠致病性大肠埃希氏菌的分离鉴定  

Isolation and Identification of Shiga Toxin Producing Escherichia coli and Enteropathogenic Escherichia coli in Beef Samples

作　　者：朱应飞 聂翔 熊丽霞 吴学平 占忠旭 匡佩琳 ZHU Yingfei;NIE Xiang;XIONG Lixia;WU Xueping;ZHAN Zhongxu;KUANG Peilin(Jiangxi General Institute of Testing and Certification Food Testing Institute,Nanchang 330000,China)

机构地区：[1]江西省检验检测认证总院食品检验检测研究院,江西南昌330000

出　　处：《食品安全导刊》2023年第28期121-124,共4页China Food Safety Magazine

基　　金：江西省市场监督管理局科技项目(GSJK202104);江西省检验检测认证总院科研项目(ZYK202212)。

摘　　要：于2018年9月—2019年3月,每个月从超市和农贸市场中抽取20个样本,共抽取140个牛肉及制品样本,在qPCR初筛时stx阳性率为45.8%,eae的阳性率为52.2%。每月的stx初筛阳性率分别为65%、60%、80%、5%、25%、45%和40%;eae初筛阳性率分别为70%、70%、65%、0%、40%、65%和55%。检出携带stx基因的产类志贺毒素大肠埃希氏菌样本4份,样本阳性菌株检出率为2.9%;检出携带eae基因的肠致病性大肠埃希氏菌样本12份,样本阳性菌株检出率为8.6%。From September 2018 to March 2019,20 samples were taken from supermarkets and farmers’markets every month,with a total of 140 beef and product samples taken.the positive rate of stx was 45.8%,and the positive rate of eae was 52.2%.The monthly positive rates of stx initial screening positive rates of 65%,60%,80%,5%,25%,45%,and 40%,respectively;The positive rates of eae initial screening were 70%,70%,65%,0%,40%,65%,and 55%,respectively.Four Shiga toxin producing Escherichia coli samples carrying the stx gene were detected,with a positive strain detection rate of 2.9%;From September 2018 to March 2019,12 samples of enteropathogenic Escherichia coli carrying the eae gene were detected,with a positive strain detection rate of 8.6%.

关 键 词：产志贺毒素大肠埃希氏菌 肠致病性大肠埃希氏菌 分离 鉴定 

分 类 号：TS251.1[轻工技术与工程—农产品加工及贮藏工程]