结肠癌患者血清miR-370-3p、ANO1表达变化及其临床意义  

Expression changes of serum miR-370-3p and ANO1 in colon cancer patients and the clinical significance

作　　者：张乐[1] 李昊泽 刘凯东[1] 白月奎[1] ZHANG Le;LI Haoze;LIU Kaidong;BAI Yuekui(Department of General Surgery,Beijing Haidian Hospital,Beijing 100091,China)

机构地区：[1]北京市海淀医院普外科,北京100091

出　　处：《山东医药》2023年第31期19-23,共5页Shandong Medical Journal

基　　金：北京市外籍高层次人才资助计划项目(J202011)。

摘　　要：目的探讨血清微小RNA-370-3p(miR-370-3p)、茴香胺1(ANO1)表达与结肠癌病理特征及术后复发的关系。方法选取94例接受腹腔镜手术治疗的结肠癌患者为结肠癌组、50例结肠息肉患者为良性病变组、50名健康体检者为对照组。用实时荧光定量PCR法检测各组血清miR-370-3p、ANO1表达。术后随访3年,复发31例(复发组)、未复发63例(未复发组)。在线网站TargetScan预测miR-370-3p与ANO1的靶向关系,用Pearson相关法分析结肠癌患者血清miR-370-3p与ANO1 mRNA表达的相关性;多因素Logistic回归分析结肠癌患者术后复发的影响因素;用受试者工作特征(ROC)曲线分析血清miR-370-3p、ANO1 mRNA对结肠癌患者术后复发的预测价值。结果结肠癌组血清miR-370-3p表达低于良性病变组、对照组(P均<0.05),ANO1 mRNA表达高于良性病变组、对照组(P均<0.05),良性病变组与对照组血清miR-370-3p、ANO1 mRNA表达比较差异均无统计学意义(P均>0.05)。在线网站TargetScan预测miR-370-3p与ANO1在649~655和676~682处有结合位点,结肠癌患者血清miR-370-3p与ANO1 mRNA中表达呈负相关(r=-0.609,P<0.05)。血清miR-370-3p、ANO1 mRNA表达在不同分化程度、TNM分期、侵犯浆膜、淋巴结转移结肠癌患者中差异有统计学意义(P均<0.05)。TNM分期Ⅲ~Ⅳ期、侵犯浆膜、淋巴结转移和ANO1 mRNA≥1.64为术后复发的独立危险因素,miR-370-3p≥0.74为独立保护因素(P均<0.05)。ROC曲线分析显示,血清miR-370-3p、ANO1 mRNA联合预测结肠癌患者术后复发的曲线下面积(0.876)大于二者单独预测的曲线下面积(0.786、0.783),比较差异有统计学意义(P均<0.05)。结论结肠癌患者血清miR-370-3p低表达、ANO1 mRNA高表达,与肿瘤分化程度、TNM分期、侵犯浆膜、淋巴结转移和术后复发有关,二者联合预测结肠癌患者术后复发的价值较高。Objective To investigate the relationships between serum microRNA-370-3p(miR-370-3p)and anoctamin 1(ANO1)expression and the pathological characteristics of colon cancer and recurrence after laparoscopic surgery.Methods Ninety-four cases of colon cancer patients undergoing laparoscopic surgery(the colon cancer group),50 cases of colon polyp patients(the benign lesion group),and 50 cases of healthy individuals(the control group)were selected.Real-time fluorescence quantitative polymerase chain reaction was used to detect serum miR-370-3p and ANO1 mRNA expression of each group.Patients were followed up for 3 years after laparoscopic surgery;there were 31 cases of recurrence(recurrence group)and 63 cases of non-recurrence(non recurrence group).Online website TargetScan was used to predict the targeting relationship between miR-370-3p and ANO1,and Pearson correlation method was used to analyze the correlation between serum miR-370-3p and ANO1 mRNA expression in colon cancer patients.Multivariate Logistic regression analysis was used to analyze the influencing factors for postoperative recurrence in colon cancer patients after laparoscopic surgery.The value of serum miR-370-3p and ANO1 mRNA in predicting postoperative recurrence in colon cancer patients was ana lyzed using receiver operating characteristic(ROC)curves.Results The expression of serum miR-370-3p in the colon cancer group was lower than that in the benign lesion group and control group(both P<0.05),and the expression of ANO1 mRNA was higher than that in the benign lesion group and control group(both P<0.05).There were no statistically significant differences in serum miR-370-3p or ANO1 mRNA between the benign lesion group and the control group(both P>0.05).The online website TargetScan predicted that there were binding sites between miR-370-3p and ANO1 at 649-655 and 676-682,and there was a negative correlation between the expression of miR-370-3p and ANO1 mRNA in the colon cancer patients(r=-0.609,P<0.05).There were significant differences in serum miR-37

关 键 词：结肠癌 微小RNA-370-3p 茴香胺1 临床病理特征 复发 

分 类 号：R735.3[医药卫生—肿瘤]