宫颈癌组织中BAG3蛋白表达水平及意义  

Expression level of BAG3 protein in cervical cancer tissue and its significance

作　　者：冯宁 刘艳[1] FENG Ning;LIU Yan(Department of Obstetrics and Gynecology,Xi′an Central Hospital,Xi′an,Shaanxi 710061,China)

机构地区：[1]陕西省西安市中心医院妇产科,陕西西安710061

出　　处：《检验医学与临床》2023年第21期3152-3155,3159,共5页Laboratory Medicine and Clinic

摘　　要：目的分析Bcl-2相关抗凋亡基因3(BAG3)在宫颈癌组织中的表达水平及意义。方法选取2016年10月至2018年10月该院收治的201例研究对象的宫颈组织标本为研究对象,其中宫颈癌组织标本70例(宫颈癌组)、低级别鳞状上皮内瘤变(LSIL)组织标本46例(LSIL组)、高级别鳞状上皮内瘤变(HSIL)组织标本50例(HSIL组)和宫颈正常组织标本35例(正常组)。采用免疫组织化学染色法检测所有标本组织中BAG3蛋白表达水平,分析不同临床病理特征宫颈癌患者BAG3蛋白表达差异,采用多因素Cox回归分析影响宫颈癌患者预后的因素。结果BAG3蛋白在宫颈癌组中的高表达率>HSIL组>LSIL组>正常组(P<0.05);低分化、淋巴结转移宫颈癌患者的BAG3蛋白高表达率分别高于高/中分化、无淋巴结转移患者(P<0.05);70例宫颈癌患者3年生存率为60.00%,K-M生存曲线显示BAG3高表达患者3年生存率低于BAG3低表达患者,差异有统计学意义(Log-rankχ^(2)=4.821,P=0.023)。不同分化程度、有无淋巴结转移、不同BAG3蛋白表达水平的宫颈癌患者3年生存率差异有统计学意义(P<0.05)。多因素Cox分析结果显示,低分化、有淋巴结转移、BAG3蛋白高表达均是影响宫颈癌患者预后的独立危险因素(P<0.05)。结论BAG3蛋白在宫颈癌组织中呈高表达,其表达水平与宫颈癌分化程度、淋巴结转移有关,BAG3蛋白高表达是宫颈癌预后的危险因素,有望作为诊治宫颈癌的有效生物标志物。Objective To investigate the expression level of Bcl-2 associated anti-apoptosis gene 2(BAG3)in cervical cancer tissue and its significance.Methods From October 2016 to October 2018,201 cervical tissue specimens from Xi′an Central Hospital were selected as the research objects,including 70 cervical cancer tissue specimens(cervical cancer group),46 low-grade squamous intraepithelial lesion tissue specimens(LSIL group),50 samples of high-grade squamous intraepithelial lesions(HSIL group)and 35 samples of normal cervical tissue(normal group).Immunohistochemical staining method was used to detect the expression level of BAG3 protein in all specimen tissues,differences in BAG3 protein in cervical cancer patients with different clinicopathological features were analyzed,and multifactorial Cox regression was used to analyze BAG3 protein expression as a factor affecting the prognosis of cervical cancer patients.Results The high expression rate of BAG3 protein in cervical cancer group>HSIL group>LSIL group>normal group(P<0.05).The high expression rate of BAG3 protein in patients with poorly differentiated and lymph node metastasis was respectively higher than that in patents with high and middle differentiated and without lymph node metastasis(P<0.05).The three-year survival rate of 70 cervical cancer patients was 60.00%,K-M survival curve showed that the 3-year survival rate of patients with high BAG3 expression was lower than that of patients with low BAG3 expression,and the difference was statistically significant(Log-rankχ^(2)=4.821,P=0.023).The differences in 3-year survival rates of cervical cancer patients with different degrees of differentiation,with or without lymph node metastasis,and with different levels of BAG3 protein were statistically significant(P<0.05).Multivariate Cox analysis showed that poor differentiation,lymph node metastasis and high BAG3 expression were independent risk factors of the prognosis of cervical cancer patients(P<0.05).Conclusion The expression of BAG3 protein in cervical cancer is

关 键 词：Bcl相关抗凋亡蛋白3 宫颈癌 癌前病变 临床意义 

分 类 号：R737.33[医药卫生—肿瘤]