东方蜜蜂微孢子虫LCFAL 2基因的分子特性及系统进化分析  

Investigation of molecular characteristics and phyletic eolution of Nosema ceranae LCFAL 2 gene

作　　者：王思懿 胡颖 刘小玉 荆欣 冯佩林 朱乐冉 姚雨桐 陈大福 郭睿 徐国钧 WANG Si-Yi;HU Ying;LIU Xiao-Yu;JING Xin;FENG Pei-Lin;ZHU Le-Ran;YAO Yu-Tong;CHEN Da-Fu;GUO Rui;XU Guo-Jun(College of Animal Sciences(College of Bee Science),Fujian Agriculture and Forestry University,Fuzhou 350002,China;Apitherapy Research Institute,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]福建农林大学动物科学学院(蜂学学院),福州350002 [2]福建农林大学蜂疗研究所,福州350002

出　　处：《环境昆虫学报》2023年第5期1284-1290,共7页Journal of Environmental Entomology

基　　金：国家自然科学基金面上项目(32172792);国家现代农业产业技术体系专项资金项目(CARS-44-KXJ7);福建省自然科学基金面上项目(2022J01131334);宁夏回族自治区重点研发项目(2022BBF02037);福建农林大学硕士生导师团队项目(郭睿);福建省大学生创新创业训练计划项目(202210389128,202210389136)。

摘　　要：本研究旨在解析东方蜜蜂微孢子虫Nosema ceranae的长链脂肪酸辅酶A连接酶(long chain fatty acid COA ligase)基因LCFAL 2的分子特性,鉴定东方蜜蜂微孢子虫和其他物种的LCFAL 2蛋白的保守基序和结构域并进行系统进化分析,以期丰富东方蜜蜂微孢子虫LCFAL 2的信息,为深入开展相关功能研究提供基础。通过Expasy网站上的相关软件预测和分析LCFAL 2的理化性质、跨膜螺旋域、信号肽、磷酸化位点、二级结构和三级结构。使用MEME软件预测东方蜜蜂微孢子虫和其他物种LCFAL 2蛋白的保守基序。采用TBtools软件预测东方蜜蜂微孢子虫和其他物种LCFAL 2的结构域。通过Mega 11.0软件构建东方蜜蜂微孢子虫和其他物种的LCFAL 2的系统进化树。结果表明,东方蜜蜂微孢子虫LCFAL 2含有1836个核苷酸,可编码611个氨基酸,分子式为C_(3145)H_(4985)N_(813)O_(904)S_(23),分子量约为69.39 kDa,脂溶系数为96.82,等电点为8.79,平均亲水系数为-0.161;不含跨膜螺旋域和典型信号肽;包含27个丝氨酸酸化位点,14个酪氨酸酸化位点及23个苏氨酸磷酸化位点;包含240个α-螺旋,124个延长链,48个β-转角及199个无规则卷曲;可同时定位于细胞核、细胞质和线粒体;另外,LCFAL 2及其模板5mst.1.A之间的同源性为21.44%。东方蜜蜂微孢子虫与蜜蜂微孢子虫Nosema apis等9个物种的LCFAL 2中均含有6个相同的保守基序。东方蜜蜂微孢子虫、管孢属微孢子虫Tubulinosema ratisbonensis、蜜蜂微孢子虫、巨蟹肠孢菌Enterospora canceri、烟粉虱Spraguea lophii、毕氏肠细胞虫Enterocytozoon bieneusi、东方赤孢子虫Hamiltosporidium tvaerminnensis和褶皱臂尾轮虫Brachionus plicatilis的LCFAL 2均包含AFD_class_I superfamily结构域。东方蜜蜂微孢子虫LCFAL 2(XP_024331795.1)与蜜蜂微孢子虫LCFAL 2(EQB61677.1)聚为一支,进化距离最近。研究结果明确了东方蜜蜂微孢子虫LCFAL 2的分子特性,并揭示LCFAL 2可能是亲�This study aims to decipher the molecular characteristics of long chain fatty acid COA ligase 2 gene LCFAL 2 in Nosema ceranae,identify conserved motifs and structural domains and conduct phylogenetic analysis of LCFAL 2 proteins in N.ceranae and other species,thus enriching information about N.ceranae LCFAL 2 gene and providing a foundation for related functional study.Physical and chemical property,transmembrane domain,signal peptide,phosphorylation site,secondary and tertiary structures were predicted and analyzed using relevant software on Expasy website.MEME software was emplyed to identify conserved motifs within LCFAL 2 in N.ceranae and other species.Conserved domains within LCFAL 2 were predicted with TBtools software.Mega 11.0 software was utilized to construct the phylogenetic tree with neighbor-joining method based on LCFAL 2 proteins in N.ceranae and other species.The results demonstrated that N.ceranae LCFAL 2 contained 1836 nucleotides,which can encode 611 amino acids,the molecular formula was C_(3145)H_(4985)N_(813)O_(904)S_(23),the molecular weight was about 69.39 kDa,the lipid solubility coefficient was 96.82,the isoelectric point was 8.79,and the average hydrophilic coefficient was-0.161;LCFAL 2 contained no transmembrane domain and signal peptide;LCFAL 2 included 27 serine phosphorylation sites,14 tyrosine phosphorylation sites and 23 threonine phosphorylation sites;LCFAL 2 included 240α-helix,124 extension,48β-turn and 199 random coil;the homology between LCFAL 2 and its target 5mst.1.A was 21.44%;additionally,LCFAL 2 were simultaneously localized in nucleus,cytoplasm and mitochondria.Six same conserved motifs were included in LCFAL 2 proteins in nine species such as N.ceranae and N.apis.Also,AFD_class_I superfamily structural domain were identified in LCFAL 2 in N.ceranae,Tubulinosema ratisbonensis,N.apis,Encephalitozoon cuniculi,Spraguea lophii,Enterocytozoon bieneusi,Hamiltosporidium tvaerminnensis and Brachionus plicatilis.LCFAL 2(XP_024331795.1)in N.ceranae and LCFAL 2(EQB61677.1)were c

关 键 词：东方蜜蜂微孢子虫 长链脂肪酸辅酶A连接酶2 分子特性 保守性 系统进化 

分 类 号：Q963[生物学—昆虫学] S433[农业科学—农业昆虫与害虫防治]