肾细胞癌组织中GPX4的表达及其对ACHN细胞增殖、迁移和侵袭的影响  

The expression of GPX4 in renal cell carcinoma tissues and its impact on the proliferation,migration and invasion ability of ACHN cells

作　　者：韩静雅[1] 段亚涛 张晓宇[2] 任宗涛[2] HAN Jingya;DUAN Yatao;ZHANG Xiaoyu;REN Zongtao(Department of Nuclear Medicine,the Fourth Hospital of Hebei Medical University,Hebei Shijiazhuang 050011,China;Department of Urology,the Fourth Hospital of Hebei Medical University,Hebei Shijiazhuang 050011,China)

机构地区：[1]河北医科大学第四医院核医学科,河北石家庄050011 [2]河北医科大学第四医院泌尿外科,河北石家庄050011

出　　处：《现代肿瘤医学》2023年第22期4105-4109,共5页Journal of Modern Oncology

基　　金：河北省重点科技研究计划(编号:20230758)。

摘　　要：目的:探讨肾细胞癌(renal cell carcinoma,RCC)组织及ACHN细胞中谷胱甘肽过氧化物酶4(glutathione peroxidase 4,GPX4)的表达及其对ACHN细胞增殖、迁移、侵袭能力的影响。方法:采用免疫组化法、qRT-PCR法检测RCC组织及正常肾组织中GPX4的表达水平;构建敲低GPX4的shRNA质粒并转染ACHN细胞进行功能实验,检测敲低GPX4对ACHN细胞恶性生物学行为的影响,Western blot法检测敲低GPX4对ACHN细胞GPX4蛋白表达的影响。结果:RCC组织中GPX4蛋白和mRNA的表达量明显高于正常肾组织(P<0.01),向ACHN细胞中转染shGPX4能够敲低GPX4的基因和蛋白表达(P<0.05),敲低GPX4可明显抑制ACHN细胞的增殖(P<0.05)、迁移(P<0.05)和侵袭能力(P<0.05)。结论:GPX4在RCC组织及ACHN细胞中高表达,敲低GPX4可抑制ACHN细胞的恶性生物学行为,提示GPX4可能作为一种癌基因在肾癌中发挥作用。Objective:To explore the expression of glutathione peroxidase 4(GPX4)in renal cell carcinoma(RCC)tissues and ACHN cells,also to investigate its effect on the proliferation,migration and invasion of ACHN cells.Methods:The expression of GPX4 in RCC tissues and normal renal tissues was assessed by immunohistochemistry and reverse transcription-quantitative real-time PCR(qRT-PCR).shRNA plasmid with GPX4 knockdown was constructed and transfected into ACHN cells for functional experiments to detect the effect of GPX4 knockdown on the malignant biological behavior of ACHN cells.The effect of GPX4 knockdown on the expression of GPX4 protein in ACHN cells was detected by Western blot.Results:The expression of GPX4 protein and mRNA in RCC tissues was significantly higher than that in normal renal tissues(P<0.01).Transfection of shGPX4 into ACHN cells could knock down GPX4 gene and protein expression(P<0.05).Functional experiments demonstrated that proliferation(P<0.05),migration(P<0.05)and invasion(P<0.05)ability was significantly inhibited by knockdown of GPX4 in ACHN cells.Conclusion:GPX4 was highly expressed in RCC tissues and ACHN cells and knockdown of GPX4 could inhibit the malignant biological behavior of ACHN cells,we preliminarily inferred that GPX4 may serve as an oncogene in RCC progression.

关 键 词：肾癌 GPX4 ACHN细胞 增殖 迁移 侵袭 

分 类 号：R737.11[医药卫生—肿瘤]