基质金属蛋白酶抑制剂对人胰腺癌细胞迁移和侵袭的抑制作用  

Anti-migration and invasion effect of matrix metalloproteinase inhibitor in human pancreatic cancer cells

作　　者：王建强[1] 邱爱燕[1] 张海文 吴俊本[1] 成丕光[1] 徐怀勇[1] WANG Jianqiang;QIU Aiyan;ZHANG Haiwen;WU Junben;CHENG Piguang;XU Huaiyong(Binzhou People's Hospital,Shandong Binzhou 256600,China)

机构地区：[1]滨州市人民医院,山东滨州256600

出　　处：《现代肿瘤医学》2023年第22期4153-4157,共5页Journal of Modern Oncology

基　　金：滨州市人民医院院级科研课题(编号:YBKTZR202106)。

摘　　要：目的:探讨MMI-166对人胰腺癌细胞PANC-1和SW1990迁移和侵袭的影响。方法:培养人胰腺癌细胞PANC-1、SW1990,使用不同浓度MMI-166进行干预,细胞划痕实验和Transwell实验检测细胞迁移和侵袭能力。比较各组PANC-1及SW1990细胞中MMP-2及MMP-9蛋白表达量。结果:与对照组相比,通过细胞划痕实验和Transwell实验,发现MMI-166对胰腺癌细胞系的细胞迁移能力和侵袭能力,随着浓度增加而下降明显,差异有统计学意义(P<0.05);Western blot及免疫荧光显示,MMI-166对胰腺癌细胞系MMP-2及MMP-9蛋白的表达成显著抑制作用,且存在剂量依赖性,差异有统计学意义(P<0.05)。结论:MMI-166通过特异性抑制MMP-2和MMP-9表达,在胰腺癌细胞的迁移和侵袭调控中发挥重要作用。Objective:To investigate the effects of MMI-166 on the migration and invasion of human pancreatic cancer cell lines PANC-1 and SW1990.Methods:PANC-1,SW1990 cells were cultured in vitro,which were treatment with different concentrations of MMI-166.Cell migration and invasion were detected by scratch assay and Transwell assay.The expressions of proteins of MMP-2 and MMP-9 were compared in different groups of PANC-1 and SW1990 cells.Results:Compared with the control group,the scratch assay and Transwell assay showed that MMI-166 decreased cell migration and invasion with increasing concentration,with statistical difference(P<0.05).Western blot and immunofluorescence showed MMI-166 significantly inhibited the expression of MMP-2 and MMP-9 protein in a dose-dependent manner,with statistical difference(P<0.05).Conclusion:MMI-166 inhibited the MMP-2 and MMP-9 activity in pancreatic cancer cells and it is useful for the regulation of pancreatic cancer cells migration and invasion.

关 键 词：胰腺癌 迁移 侵袭 基质金属蛋白酶 MMI-166 

分 类 号：R735.9[医药卫生—肿瘤]