BTF3在口腔鳞状细胞癌组织中的表达及其与PINCH的相关性  

Expression of BTF3 in oral squamous cell carcinoma tissues and its correlation with PINCH

作　　者：胡洁 高峰[2] 王云静 修小斐 王大维[1] HU Jie;GAO Feng;WANG Yunjing;XIU Xiaofei;WANG Dawei(Department of Oral and Maxillofacial Surgery,the Third Hospital of Hebei Medical University,Hebei Shijiazhuang 050000,China;Department of Pathology,the Third Hospital of Hebei Medical University,Hebei Shijiazhuang 050000,China)

机构地区：[1]河北医科大学第三医院口腔颌面外科,河北石家庄050000 [2]河北医科大学第三医院病理科,河北石家庄050000

出　　处：《现代肿瘤医学》2023年第22期4162-4166,共5页Journal of Modern Oncology

基　　金：河北省财政厅资助临床医学优秀人才培养项目(编号:冀卫办科教[2021]1号)。

摘　　要：目的:探讨基础转录因子3(basic transcription factor 3,BTF3)在口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)组织中的表达及其与PINCH的相关性。方法:采用免疫组织化学法检测BTF3在73对OSCC组织及相邻癌旁正常组织(45例伴淋巴结转移)中的表达,并通过肿瘤基因组图谱(the Cancer Genome Atlas,TCGA)数据库分析BTF3、PINCH在OSCC组织及相邻癌旁正常组织中的表达以验证免疫组织化学检测结果。分析BTF3表达与患者年龄、性别、肿瘤分化程度及淋巴结转移等临床病理参数的关系及其与PINCH在OSCC中表达的相关性。结果:免疫组织化学检测结果显示,BTF3在OSCC组织中的表达明显高于相邻癌旁正常组织(60.27%vs 39.73%,P=0.013);TCGA数据库分析显示,OSCC组织中BTF3、PINCH的阳性表达率均显著高于二者在相邻癌旁正常组织中的阳性表达率。在OSCC中,BTF3在淋巴结转移组中的阳性表达率明显高于其在无淋巴结转移组中的阳性表达率(71.11%vs 42.86%,P=0.016);BTF3在低分化组OSCC中的阳性表达率明显高于其在高分化组OSCC中的阳性表达率(71.43%vs 45.16%,P=0.023);BTF3的表达与患者性别、年龄、肿瘤大小和部位无相关性(P>0.05);BTF3和PINCH在OSCC中的表达呈正相关(r=0.286,P=0.015)。结论:BTF3与OSCC的发生、发展及淋巴结转移有关,有望成为判断OSCC发生、发展及预测淋巴结转移的潜在分子标志物,其机制可能与PINCH正协同作用有关。Objective:To investigate the expression of basic transcription factor 3(BTF3)and its correlation with PINCH in oral squamous cell carcinoma(OSCC)tissues.Methods:The expression of BTF3 in 73 pairs of OSCC carcinomas and adjacent normal tissues(45 cases with lymph node metastasis)was detected by immunohistochemistry.The expression of BTF3 and PINCH in OSCC cancer and adjacent normal tissues was analyzed by the Cancer Genome Atlas(TCGA)database to verify the results of immunohistochemistry.To analyze the relationship between BTF3 expression and clinicopathological parameters such as age,gender,tumor differentiation and lymph node metastasis of patients and its correlation with PINCH expression in OSCC.Results:The results of immunohistochemistry showed that the expression of BTF3 in OSCC cancer tissues was significantly higher than that in adjacent normal tissues(60.27%vs 39.73%,P=0.013).TCGA database analysis showed that the positive expression rates of BTF3 and PINCH in OSCC cancer tissues were significantly higher than that of both in adjacent normal tissues.In OSCC,the positive expression rate of BTF3 in the lymph node metastasis group was significantly higher than that in the no lymph node metastasis group(71.11%vs 42.86%,P=0.016).The positive expression rate of BTF3 in the hypodifferentiated OSCC group was significantly higher than that in the highly differentiated OSCC group(71.43%vs 45.16%,P=0.023).BTF3 expression did not correlate with patient gender,age,tumour size and site(P>0.05).BTF3 and PINCH expression in OSCC were positively correlated(r=0.286,P=0.015).Conclusion:BTF3 is correlated with the tumorigenesis,development and lymph node metastasis of OSCC,and may be a potential molecular marker for determining the occurrence and development of OSCC and predicting lymph node metastasis,and its mechanism may be related to the positive synergistic effect of PINCH.

关 键 词：BTF3 PINCH 口腔鳞状细胞癌 免疫组织化学 TCGA数据库 

分 类 号：R739.8[医药卫生—肿瘤]