红外热成像在大鼠腰椎间盘突出造模中的应用及其意义  

Application and Significance of Infrared Thermal Imaging in Modeling Lumbar Disc Herniation in Rats

作　　者：吴思[1,2] 王平 张晓宇[1,2] 刘爱峰 符碧峰[1,2] 苏瑾 张起[3] 谢海波 WU Si;WANG Ping;ZHANG Xiaoyu;LIU Aifeng;FU Bifeng;SU Jin;ZHANG Qi;XIE Haibo(First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300381,China;National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion,Tianjin 300381,China;Tianjin Binhai New Area Hospital of Traditional Chinese Medicine,Tianjin 300451,China)

机构地区：[1]天津中医药大学第一附属医院,天津300381 [2]国家中医针灸临床医学研究中心 [3]天津市滨海新区中医医院

出　　处：《中国中医骨伤科杂志》2023年第10期1-5,11,共6页Chinese Journal of Traditional Medical Traumatology & Orthopedics

基　　金：天津市科技计划项目(21JCQNJC01690);王平劳模创新工作室——天津市教委资助项目(津教工〔2016〕3号);国家中医药管理局中医药循证能力建设项目(2019XZZX-GK005);天津中医药大学第一附属医院“拓新工程”基金科研课题(院201711,院201919);天津市卫生健康科技项目(TJWJ2022QN107)。

摘　　要：目的:观察红外热成像技术在大鼠腰椎间盘突出模型建立过程中的作用及意义。方法:选择雄性清洁健康级SD大鼠20只,7周龄,体重为230~250 g。对大鼠编号后,随机分为模型组与对照组,模型组取尾椎间盘,通过手术方式植入腰4/5节段椎管内,制作腰椎间盘突出模型;对照组仅取尾椎间盘,切开分离腰部竖脊肌至椎板,不植入髓核。分别于造模后第3,7,14,21,28天观察大鼠神经功能评分、热刺激下第一次咬左足时间及1 min内咬左足次数。选择大鼠腰部、左右下肢、左右足底作为观察“热窗”,使用红外热像仪,对造模后第3,7,14,21,28天大鼠相关部位进监测,记录造模后肢体温度变化情况。结果:模型组在造模后各个时间点神经功能评分与对照组差异均有统计学意义(P<0.05);造模后第3,7,14天模型组与对照组在首次缩左足时间上差异有统计学意义(P<0.05)。造模后第21天及第28天,模型组首次缩左足时间较第3,7,14天差异有统计学意义(P<0.05)。造模后第3天及第21天模型组与对照组在缩左足次数上差异有统计学意义(P<0.05)。造模后第3,7,14天模型组左下肢、左足底温度较对照组差异有统计学意义(P<0.05),造模后第21天和第28天模型组左下肢、左足底温度较对照组差异无统计学意义(P>0.05);模型组腰部、右下肢、右足底温度较对照组在造模后第3,7,14,21,28天差异无统计学意义(P>0.05)。结论:大鼠腰椎间盘突出症模型建立的第3,7,14天,造模侧肢体及足底温度降低,红外热成像技术可作为模型评价的手段之一。Objective:To observe the effect and significance of infrared thermal imaging in the process of establishing the model of lumbar disc herniation in rats.Methods:20 male clean healthy SD rats,7 weeks of age and weighting 230250 g,were selected.After the rats were numbered,they were randomly divided into model group and control group.In the model group,the caudal disc was removed and surgically implanted into the lumbar canal at 4/5 segments to create a lumbar disc herniation model.In the control group,only the caudal disc was removed,and the lumbar erectus spinalis muscle was cut and separated from the lamina,without nucleus pulposus implantation.Neurological function scores,the first time of biting the left foot and the number of biting the left foot within 1min were observed at 3,7,14,21 and 28 d after modeling,respectively.The waist,left and right lower limbs,left and right soles of the feet of rats were selected as“hot windows”for observation.The relevant parts of the rats at 3,7,14,21 and 28 d after modeling were monitored by infrared thermal imager,and the temperature changes of the limbs after modeling were recorded.Results:The neurological function scores of the model group and the control group were statistically different at each time point after modeling(P<0.05).On the 3,7 and 14 d after modeling,the time of first left foot retraction between the model group and the control group was statistically different(P<0.05).On the 21 and 28 d after modeling,the time of first left foot retraction in the model group was statistically different from that on the 3,7 and 14 d after modeling(P<0.05).On the 3 and 21 d after modeling,there was a difference in the number of left foot retraction between the model group and the control group(P<0.05).There were statistical differences in the left lower limb and left plantar temperature of the model group compared with the control group on the 3,7 and 14 d after modeling(P<0.05),but there were no statistical differences in the left lower limb and left plantar temperature

关 键 词：大鼠 腰椎间盘突出症 模型 红外热成像 

分 类 号：R-33[医药卫生]