温阳补肾方对激素性股骨头坏死模型兔血清中成骨、成血管因子及H型血管标志物的影响  

Effect of Wenyang Bushen Formula on Osteogenic and Angiogenic Factors and H-type Vascular Markers in Rabbit Serum in a Model of Hormone-Induced Femoral Head Necrosis

作　　者：宋红梅[1] 谢文博 林菲菲 吴毅泽 王和鸣[2] SONG Hongmei;XIE Wenbo;LIN Feifei;WU Yize;WANG Heming(The Second People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou 350003,China;Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;Fuzhou Changle District Hospital of Traditional Chinese Medicine,Fuzhou 350299,China)

机构地区：[1]福建中医药大学附属第二人民医院,福州350003 [2]福建中医药大学 [3]福州市长乐区中医院

出　　处：《中国中医骨伤科杂志》2023年第10期6-11,共6页Chinese Journal of Traditional Medical Traumatology & Orthopedics

基　　金：国家自然科学基金项目(81973879)。

摘　　要：目的:观察温阳补肾方对兔激素性股骨头坏死血清中的成血管因子低氧诱导因子1α(HIF-1α)、血管内皮生长因子(VEGF),成骨因子骨形态发生蛋白2(BMP2)、成骨细胞特异基因(Osterix)及H型血管标志物血小板-内皮细胞黏附分子(CD31)、内皮黏蛋白(EMCN)的影响,探讨温阳补肾方逆转激素性股骨头坏死的作用机制。方法:选用27只健康雄性新西兰大白兔,随机分成正常组、模型组、中药组(温阳补肾方临床等效剂量组)、基因沉默组(miR-130a抑制剂)。在造模成功后,从正常组中随机抽取1只,造模组中随机抽取2只,用于模型鉴定。采用ELISA试剂盒检测兔血清中低氧诱导因子、血管内皮生长因子、骨形态发生蛋白2、Osterix、CD31、内皮黏蛋白浓度。结果:兔血清中低氧诱导因子、血管内皮生长因子、骨形态发生蛋白2、Osterix、CD31、内皮黏蛋白的浓度,模型组明显低于正常组,差异有统计学意义(P<0.05);中药组明显高于模型组,差异有统计学意义(P<0.05);基因沉默组明显低于正常组和中药组,差异有统计学意义(P<0.05)。结论:大量糖皮质激素的应用可下调血管内皮生长因子、低氧诱导因子、骨形态发生蛋白2、Osterix、CD31、内皮黏蛋白的表达,从而造成骨质破坏和血供受损,导致股骨头坏死。温阳补肾方能调控成血管相关因子血管内皮生长因子、低氧诱导因子,成骨相关因子骨形态发生蛋白2、Osterix,H型血管标志物CD31、内皮黏蛋白,使以上各项指标的浓度均增高,促使股骨头内部新生血管增多,增强股骨头内营养供给,从而增强骨组织的自我修复能力,这可能是温阳补肾方能够逆转激素性股骨头坏死的原因之一。Objective:To observe the effects of Wenyang Bushen formula on the serum of hypoxia inducible factor-1α(HIF-1α),vascular endothelial growth factor(VEGF),osteogenic factor bone morphogenetic protein-2(BMP2),osterix and H-type vascular markers platelet endothelial cell adhesion molecule-1(CD31)and Endomucin(EMCN)in rabbits with hormone-induced femoral head necrosis,and to explore the mechanism of Wenyang Bushen formula in correcting and reversing hormone-induced femoral head necrosis.Methods:27 healthy male New Zealand white rabbits were randomly divided into normal group,model group,traditional Chinese medicine group(clinical equivalent dose group of Wenyang Bushen prescription)and gene silencing group(miR-130a inhibitor).After successful modeling,1 animal was randomly selected from the normal group and 2 animals were randomly selected from the modeling group for model identification.The concentrations of HIF-1α,VEGF,BMP2,osterix,CD31 and EMCN in rabbit serum were determined by ELISA kit.Results:The concentrations of HIF-1α,VEGF,BMP2,osterix,CD31 and EMCN in rabbit serum were significantly lower in model group than that in normal group(P<0.05),significantly higher in traditional Chinese medicine group than that in model group(P<0.05),and significantly lower in silent group than that in normal group and traditional Chinese medicine group(P<0.05).Conclusion:The use of a large number of glucocorticoids could down-regulate the expression of VEGF,HIF-α,BMP2,Osterix,CD31,and EMCN,further cause bone destruction and impaired blood supply,and lead to osteonecrosis of femoral head.Wenyang Bushen formula can regulate angiogenesis-related factors VEGF and HIF-α,osteogenesis-related factors BMP2 and Osterix,and H-type vascular markers CD31 and EMCN,increase the concentrations of the above indicators,and angiogenesis in the femoral head,enchance the internal nutrition supply of femoral head,thereby enhancing the self-repair ability of bone tissue,redress the lesion,which may be one of the mechanisms of Wenyang Bushen formu

关 键 词：激素性股骨头坏死 温阳补肾方 血小板-内皮细胞黏附分子 内皮黏蛋白 低氧诱导因子 血管内皮生长因子 骨形态发生蛋白2 成骨细胞特异基因 

分 类 号：R-33[医药卫生]