不同剂量异氟烷麻醉对新生大鼠认知功能的影响及机制  

Effects and mechanism of different doses of isoflurane anesthesia on cognitive function of neonatal rats

作　　者：缪文禹 任灵[2] 毛舜 佟昌慈 MIAO Wen-yu;REN Ling;MAO Shun;TONG Chang-ci(Department of Surgery and Anesthesiology,Liaoning Provincial Jin Qiu Hospital,Shenyang 110016;Department of Pediatrics,The Second Affiliated Hospital of Shenyang Medical College,Shenyang 110002;Shuren International College of Shenyang Medical College,Shenyang 110031,China)

机构地区：[1]辽宁省金秋医院手术麻醉科,辽宁沈阳110016 [2]沈阳医学院附属第二医院儿科学,辽宁沈阳110002 [3]沈阳医学院树人国际学院,辽宁沈阳110031

出　　处：《解剖科学进展》2023年第4期345-347,共3页Progress of Anatomical Sciences

基　　金：辽宁省自然科学基金(201602771,2020-MS-311)。

摘　　要：目的探讨不同剂量异氟烷全身麻醉对新生大鼠认知功能的影响及可能机制。方法24只出生后7 d SD大鼠,随机分为对照组、1.0%异氟烷处理组、1.5%异氟烷处理组和2.0%异氟烷处理组。各组大鼠于21d后行旷场实验,35d后行Morris水迷宫检测。HE染色观察各组大鼠海马组织病理学改变;Western blot方法检测大鼠海马组织Ki-67、神经元核心抗原(NeuN)增殖、NLRP3和caspase-1蛋白的表达。结果与对照组相比,1.0%、1.5%或2.0%异氟烷处理组大鼠海马Ki-67和Neu N蛋白表达均明显降低(P<0.05),异氟烷麻醉各组间无显著性差异。与对照组相比,1.0%、1.5%或2.0%异氟烷处理后,大鼠逃避潜伏期、目标象限时间和穿越平台次数均明显延长;大鼠海马发生一定程度的损伤;大鼠海马组织NLRP3、caspase-1、IL-1β和ASC蛋白表达升高(P<0.05)。结论异氟烷全身麻醉可降低新生大鼠学习能力,其机制可能与激活NLRP3和caspase-1信号通路有关。Objective To investigate the effect and mechanism of different doses of isoflurane anesthesia on cognitive function of neonatal rats.Method Twenty-four SD rats were randomly divided into control group,1.0%isoflurane treatment group,1.5%isoflurane treatment group and 2.0%isoflurane treatment group.Open field and morris water maze experiments were used to evaluate the cognitive function of rats.The histopathological changes of hippocampus were observed by HE staining.The expressions of Ki-67,NeuN,NLRP3 and caspase-1 were detected by Western blot.Results Compared with the control group,the expressions of Ki-67 and NeuN in hippocampus of rats treated with isoflurane were significantly decreased.Compared with the control group,the escape latency,target quadrant time and platform crossing times of rats in isoflurane treatment group were significantly prolonged.There was no significant difference between isoflurane treatment groups.Hippocampal injury occurred after isoflurane anesthesia in rats.The expressions of NLRP3,caspase-1,IL-1βand ASC were up-regulated in hippocampus of rats treated with isoflurane.Conclusion Isoflurane anesthesia can reduce the learning ability of newborn rats,and the mechanism may be related to the activation of NLRP3 and caspase-1 signaling pathways.

关 键 词：异氟烷 麻醉 大鼠 认知功能 

分 类 号：R749.1[医药卫生—神经病学与精神病学]