黄蜀葵花总黄酮抗氧化及抗抑郁作用研究  

Antioxidant and antidepressant effects of total flavonoids from Abelmoschus manihot(L.)Medicus flower

作　　者：唐远谋[1] 陈鹏[2] 曾黎黎 颜军[2] 何钢[2] TANG Yuanmou;CHEN Peng;ZENG Lili;YAN Jun;HE Gang(College of Food and Biological Engineering,Chengdu University,Chengdu 610106;The Key Laboratory of Medicinal and Edible Plants Resources Development of Sichuan Education Department,Sichuan Industrial Institute of Antibiotics,Chengdu University,Chengdu 610052;Sichuan Tianyuan Olive Co.,Ltd.,Dazhou 636250)

机构地区：[1]成都大学食品与生物工程学院,成都610106 [2]成都大学药食同源植物资源开发高校重点实验室四川抗菌素工业研究所,成都610052 [3]四川天源油橄榄有限公司,达州636250

出　　处：《中国食品添加剂》2023年第10期153-158,共6页China Food Additives

基　　金：四川省中医药管理局科研专项资助项目(NO.2020JC0157);固态发酵资源利用四川省重点实验室开放课题(2018GTJ009);成都大学人才培养质量和教学改革项目(cdjgb2022156);四川省国际科技合作(澳新)研究院课题(AXYJ2022-001);川菜发展研究中心项目(CC22Z14)。

摘　　要：对黄蜀葵花总黄酮抗氧化和抗抑郁作用进行研究。采用小鼠强迫游泳实验(FST)、悬尾实验(TST)、旷场实验(OFT)作为行为学实验模型考察黄蜀葵花总黄酮的抗抑郁作用;利用试剂盒检测小鼠血清中超氧化物歧化酶(SOD)活力、丙二醛(MDA)的含量考察黄蜀葵花总黄酮体内抗氧化作用;采用高效液相色谱法测定小鼠血清中色氨酸(Trp)、犬尿氨酸(Kyn)的含量及比值。结果表明:与普通组相比,黄蜀葵花总黄酮各组有效缩短了小鼠TST、FST的静止时间;黄蜀葵花总黄酮高剂量组(200 mg/kg)能有效降低小鼠血清中MDA质量含量,改善小鼠血清中SOD活力,提高小鼠血清中Trp/Kyn的比值。黄蜀葵花黄酮具有抗抑郁作用,其作用机制可能与黄酮的抗氧化作用、色氨酸与犬尿氨酸的代谢途径有关。The antioxidant and antidepressant of total flavonoids from Abelmoschus manihot(L.)Medicus flower were studied.The mice forced swimming test(FST),tail suspension test(TST)and open field test(OFT)were used as experimental behavioural models to investigate the antidepressant effect of total flavonoids of Abelmoschus manihot(L.)Medicus flower.The activity of superoxide dismutase(SOD)and the content of Malondialdehyde(MDA)in the serum of mice were detected with the kit to investigate the antioxidant effect of total flavonoids of Abelmoschus manihot in vivo;the content and ratio of tryptophan(Trp)and kynurenine(Kyn)in mice serum were determined by high-performance liquid chromatography.The results showed that,compared with the normal group,the total flavonoids of Abelmoschus manihot(L.)Medicus flower effectively shortened the static time of TST and FST in mice.The high dose group of total flavonoids from Abelmoschus manihot(L.)Medicus flower(200 mg/kg)could effectively reduce the content of MDA in serum of mice,improve the activity of SOD in serum of mice and increase the ratio of Trp/Kyn in serum of mice.The total flavonoids from Abelmoschus manihot(L.)Medicus flower had antidepressant effect,and its mechanism might be related to the antioxidant effect of flavonoids and the metabolic pathway of tryptophan and kynurenine.

关 键 词：黄蜀葵花 总黄酮 抗抑郁 色氨酸 犬尿氨酸 

分 类 号：TS201.1[轻工技术与工程—食品科学]