Dynamin 3在胃癌中的作用机制及预后价值  

The mechanism of action and prognostic value of Dynamin 3 in gastric cancer

作　　者：涂儒鸿 Gildas Eric Sita Emmanuel 钟情 郑朝辉[1] 李平[1] 谢建伟[1] 王家镔[1] 林建贤[1] 陆俊[1] 陈起跃[1] 曹龙龙[1] 林密[1] 黄昌明[1] Tu Ruhong;Gildas Eric Sita Emmanuel;Zhong Qing;Zheng Chaohui;Li Ping;Xie Jianwei;Wang Jiabin;Lin Jianxian;Lu Jun;Chen Qiyue;Cao Longlong;Lin Mi;Huang Changming(Department of Gastric Surgery,Fujian Medical University Union Hospital,Fuzhou 350001,China)

机构地区：[1]福建医科大学附属协和医院胃外科,福州350001

出　　处：《中华消化外科杂志》2023年第9期1100-1112,共13页Chinese Journal of Digestive Surgery

基　　金：福建省科技创新联合资金项目(2019Y9098);福建医科大学启航基金一般项目(2018QH1021)。

摘　　要：目的探讨Dynamin 3(DNM3)在胃癌中的作用机制及预后价值。方法采用生物信息分析、实验研究方法和回顾性队列研究方法。收集2013年1月至2018年7月福建医科大学附属协和医院收治的153例行根治性胃切除术胃癌患者的临床病理资料、新鲜胃癌及配对正常组织样本和石蜡切片,行实时荧光定量聚合酶链式反应检测、免疫印迹实验、流式细胞周期实验、免疫组织化学染色检测和预后分析。收集癌症基因组图谱(TCGA)数据库的胃腺癌(STAD)数据集进行生物信息分析。观察指标:(1)胃癌中DNM3基因在TCGA-STAD中的表达情况。(2)胃癌中DNM3的突变和拷贝数改变。(3)胃癌中DNM3的启动子甲基化水平。(4)胃癌中DNM3、p53的蛋白相对表达量。(5)胃癌中DNM3相关性和富集性分析。(6)流式细胞周期G0/G1期、S期、G2/M期的比例。(7)胃癌中免疫细胞浸润和DNM3之间的相关性。(8)免疫组织化学染色检测与临床特征之间的相关性。(9)影响胃癌患者5年总生存率的独立因素分析。正态分布的计量资料以x±s表示,多组间比较采用方差分析,进一步两两比较采用LSD法;两组间比较采用t检验;偏态分布的计量资料以M(Q1,Q3)表示,组间比较采用Mann-Whitney U检验。计数资料以绝对数或百分比表示,组间比较采用χ^(2)检验或Fisher确切概率法。配对样本采用威尔科克森符号秩检验。等级资料采用秩和检验。运用Pearson相关系数或Spearman相关系数检验两组的相关性。单因素和多因素分析采用COX比例风险回归模型。采用Kaplan-Meier法绘制生存曲线并计算生存率,采用Log-Rank检验进行生存情况分析。使用Benjamini-Hochberg错误发生率校正对P值进行调整。结果(1)胃癌中DNM3基因在TCGA-STAD中的表达情况。TCGA-STAD数据库中DNM3基因在27个肿瘤组织和配对正常组织中的表达水平分别为0.775(0.605,1.161)和1.216(0.772,1.681),两者比较,差异有统计学�Objective To investigate the mechanism of action and prognostic value of Dynamin 3(DNM3)in gastric cancer.Methods The bioinformatic analysis,experimental study and retrospective cohort study was conducted.The clinicopathological data,fresh gastric cancer tissues,paired normal tissues and the corresponding paraffin sections of 153 gastric cancer patients who underwent radical gastrectomy in Fujian Medical University Union Hospital from January 2013 to July 2018 were collected.Tissues and the corresponding paraffin sections were subjected to quanti-tative real-time polymerase chain reaction,immunoblotting assay,flow cytometric cell cycle assay and immunohistochemical staining,respectively,and clinicopathological data were used for prognostic analysis.The stomach adenocarcinoma(STAD)dataset from the Cancer Genome Atlas(TCGA)database was collected for bioinformatic analysis.Observation indicators:(1)DNM3 gene expression in TCGA-STAD in gastric cancer;(2)mutations and copy number alterations of DNM3 in gastric cancer;(3)methylation level of promoter of DNM3 in gastric cancer;(4)relative protein expression of DNM3 and p53 in gastric cancer;(5)DNM3 correlation and enrichment analysis;(6)ratio of G0/G1 phase,S phase and G2/M phase of cell cycle progression;(7)correlation between immune cell infiltration and DNM3 in gastric cancer;(8)correlation between results of immunohistochemical(IHC)staining and clinical features;(9)analysis of independent factors influencing 5-year overall survival rate of gastric cancer patients.Measurement data with normal distribution were represented as Mean±SD,and comparison among multiple groups was conducted using the ANOVA and further comparison between two groups was conducted using the LSD.Comparison between two groups was conducted using the t test.Measurement data with skewed distribution were represented as M(Q1,Q3),and comparison between groups was conducted using the Mann-Whitney U test.Count data were described as absolute numbers or percentages,and compari-son between groups was co

关 键 词：胃肿瘤 预后 Dynamin 3 肿瘤抑制因子 免疫抑制 

分 类 号：R735.2[医药卫生—肿瘤]