对苯二酚对类珠藻的生物毒性  

Bio-toxicity of hydroquinone on Aliinostoc

作　　者：闫琼 邵继海[1] 陈杰锋 冯烨[1] YAN Qiong;SHAO Jihai;CHEN Jiefeng;FENG Ye(College of Environment&Ecology,Hunan Agricultural University,Changsha,Hunan 410128,China)

机构地区：[1]湖南农业大学环境生态学院,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2023年第5期624-630,共7页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目(32071647)。

摘　　要：以从酸化稻田中分离的固氮蓝藻类珠藻(Aliinostoc sp.YYLX235)为研究对象,将类珠藻于脲酶抑制剂对苯二酚最终质量浓度分别为0.0、2.5、5.0、10.0 mg/L的培养液中培养,培养2、6 d时分别对该藻株的生长、光合作用、固氮酶活性、抗氧化酶活性和氧化损伤特征等进行分析,探究对苯二酚对农田类珠藻的生物毒性。结果表明:2.5 mg/L对苯二酚处理,短时间(2 d)内对类珠藻的生长和藻胆蛋白具有刺激效应,但随着处理时间的延长,刺激效应转变为抑制效应;对苯二酚处理对藻细胞内叶绿素a和类胡萝卜素含量的影响不明显;对苯二酚处理下类珠藻的光合系统Ⅱ电子传递效率表现敏感,造成光合系统Ⅱ反应中心电子供体侧受损,短时间(2 d)内5.0 mg/L对苯二酚处理的光合系统Ⅱ最大光化学效率仅为对照的79.1%;对苯二酚处理条件下类珠藻细胞内的过氧化氢酶(CAT)活性升高,6 d时10.0 mg/L对苯二酚处理的CAT活性是对照的9.96倍,但是藻细胞的脂质氧化程度并未因对苯二酚处理而加剧;对苯二酚对类珠藻固氮酶活性具有显著的抑制效应。In order to elucidate the biotoxicity of the urease inhibitor hydroquinone to the nitrogen-fixing cyanobacterium,Alinostoc sp.YYLX235,which was isolated from an acidified rice paddy field,the effects of hydroquinone(0.0,2.5,5.0,10.0 mg/L)on the growth,photosynthesis,nitrogen fixing enzyme activity,antioxidant enzyme activity and oxidative damage characteristics of Alinostoc sp.YYLX235 were investigated on 2,6 d.The results of concentration gradient toxicology tests showed that 2.5 mg/L hydroquinone had stimulating effects on the growth and phycobilin protein of Aliinostoc sp YYLX235 at the 2 d,but the stimulatory effect shifted to an inhibitory effect as the treatment time increased.Hydroquinone showed no significant effects on the contents of chlorophyll a and carotenoids in the cells of Aliinostoc sp.YYLX235.The electron transfer efficiency of photosystemⅡ(PSⅡ)of Aliinostoc sp.YYLX235 was sensitive to hydroquinone stress.The electron donating side of PSⅡwas severely damaged by hydroquinone,the maximum quantum yield of primary photochemistry of 5.0 mg/L hydroquinone treatment at 2 d was only 79.1%of that of the control.The activity of catalase(CAT)in the cells of Aliinostoc sp.YYLX235 was promoted by hydroquinone.The CAT activity of 10.0 mg/L hydroquinone treatment was 9.96 times that of the control at 6 d,while no oxidative damage on lipids was observed under hydroquinone stress.The activity of nitrogen fixation of Aliinostoc sp.YYLX235 was significantly inhibited by hydroquinone.

关 键 词：类珠藻 固氮蓝藻 对苯二酚 光合作用 抗氧化酶 固氮酶 

分 类 号：X171.5[环境科学与工程—环境科学] Q945.78[生物学—植物学]