Seed-Specific Expression of Apolipoprotein A-IMilano Dimer in Engineered Rice Lines  

作　　者：Serena REGGI Elisabetta ONELLI Alessandra MOSCATELLI Nadia STROPPA Matteo DELL’ANNO Kiril PERFANOV Luciana ROSSI 

机构地区：[1]Department of Veterinary Medicine and Animal Science,University of Milan,Lodi 26900,Italy [2]Department of Biosciences,University of Milan,Milan 20133,Italy [3]Plantechno Srl,Casalmaggiore 26040,Italy

出　　处：《Rice science》2023年第6期587-597,共11页水稻科学（英文版）

摘　　要：Apolipoprotein A-IMilano(ApoA-IM)has been shown to significantly reduce coronary atherosclerotic plaques.However,the preparation of cost-effective pharmaceutical formulations of ApoA-IM is limited by the high cost and difficulty of purifying the protein and producing the highly effective dimeric form.The aim of this study was to create an expression cassette that specifically drives the expression of dimeric ApoA-IM in the protein bodies of rice seeds.The ApoA-IM protein under control of the 13 kDa prolamin promoter is expressed exclusively in its dimeric form within the seeds,and immunocytochemical and immunogold analyses confirmed its expression in different caryopsis tissue such as seed coat,aleurone cell and endosperm,particularly in amyloplast and storage vacuoles.A plant-based ApoA-IM production system offered numerous advantages over current production systems,including the direct production of the most therapeutically effective dimeric ApoA-IM forms,long-term protein storage in seeds,and ease of protein production by simply growing plants.Therefore,seeds had the potential to serve as a costeffective source of therapeutic ApoA-IM.

关 键 词：apolipoprotein A-IMilano engineered plant IMMUNOFLUORESCENCE immunogold analysis RICE seed-specific promoter 

分 类 号：S511[农业科学—作物学]