球孢白僵菌催化苯甲酸合成对羟基苯甲酸液体发酵优化  

Optimization of Liquid Fermentation for Synthesis of p-Hydroxybenzoic Acid from Benzoic Acid Catalyzed by Beauveria bassiana

作　　者：刘宗求 刘雨 彭继兰 王金华 王永泽[1,2,3] 高娃 LIU Zong-qiu;LIU Yu;PENG Ji-lan;WANG Jin-hua;WANG Yong-ze;GAO Wa(Hubei Collaborative Innovation Center for Industrial Fermentation Co-constructed by Ministry of Education&Hubei Province,Wuhan 430068,China;School of Biological Engineering and Food,Hubei University of Technology,Wuhan 430068,China;Key Laboratory of Fermentation Engineering,Ministry of Education,Wuhan 430068,China)

机构地区：[1]工业发酵省部共建协同创新中心,武汉430068 [2]湖北工业大学生物工程与食品学院,武汉430068 [3]发酵工程教育部重点实验室,武汉430068

出　　处：《中国调味品》2023年第11期60-66,共7页China Condiment

基　　金：国家自然科学基金(青年项目)(31501677);企业合作项目(2020240)。

摘　　要：为提高球孢白僵菌液体发酵产对羟基苯甲酸的能力,该研究确定了液体发酵过程中最适苯甲酸添加量为5 g/L,最优碳、氮源组合为玉米淀粉20 g/L和蛋白胨10 g/L,发酵温度为28℃,初始pH值为7.5,转速为200 r/min,接种量为17.5%。按照此条件进行验证实验,对羟基苯甲酸的产量可达3.85 g/L,产率为76.69%,与理论数据(产量为3.80 g/L,产率为75.72%)较吻合,说明此次优化方案可行。相较于优化前对羟基苯甲酸产量提升了12倍。该研究为对羟基苯甲酸的微生物合成提供了技术参考。In order to improve the ability of Beauveria bassiana to produce p-hydroxybenzoic acid by liquid fermentation,in this study,it is determined that the optimum addition amount of benzoic acid in the liquid fermentation process is 5 g/L,the optimum combination of carbon and nitrogen sources is 20 g/L corn starch and 10 g/L peptone,the fermentation temperature is 28℃,the initial pH value is 7.5,the rotation speed is 200 r/min,and the inoculation amount is 17.5%.According to these conditions,it is verified that the yield of p-hydroxybenzoic acid can reach 3.85 g/L,and the productivity is 76.69%,which are in good agreement with the theoretical data(yield of 3.80 g/L,productivity of 75.72%),indicating that the optimization scheme is feasible.Compared with that before optimization,the yield of p-hydroxybenzoic acid increases by 12 times.This study has provided technical references for the microbial synthesis of p-hydroxybenzoic acid.

关 键 词：球孢白僵菌 液体发酵 对羟基苯甲酸 发酵条件优化 

分 类 号：TS201.1[轻工技术与工程—食品科学]