SV40 large T antigen-induced immortalization reprograms mouse cardiomyocyte progenitors with mesenchymal stem cell characteristics and osteogenic potential  

作　　者：Yichun Yu Jiamin Zhong Connie Chen Yannian Gou Guozhi Zhao Piao Zhao Yonghui Wang Wei Zeng Annie Wang William D.Wagstaff Jr Rex C.Haydon Tong-Chuan He Russell R.Reid Michael J.Lee Hue H.Luu Jiaming Fan 

机构地区：[1]Emergency Department,Beijing Hospital,and The National Center of Gerontology,Institute of Geriatric Medicine,Chinese Academy of Medical Sciences,Beijing 100051,China [2]Molecular Oncology Laboratory,Department of Orthopaedic Surgery and Rehabilitation Medicine,The University of Chicago Medical Center,Chicago,IL 60637,USA [3]Ministry of Education Key Laboratory of Diagnostic Medicine,and the School of Laboratory Diagnostic Medicine,Chongqing Medical University,Chongqing 400016,China [4]Departments of Orthopaedic Surgery and Urology,The First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China [5]Department of Clinical Laboratory Medicine,Shanghai Jiaotong University School of Medicine,Shanghai 200025,China [6]Department of Neurology,The Second Affiliated Hospital of Jianghan University,Wuhan,Hubei 430050,China [7]Laboratory of Craniofacial Suture Biology and Development,Department of Surgery Section of Plastic Surgery,The University of Chicago Medical Center,Chicago,IL 60637,USA

出　　处：《Genes & Diseases》2023年第4期1161-1164,共4页基因与疾病（英文）

基　　金：supported in part by research grants from the National Institutes of Health(No.CA226303 to T.C.H.,No.DE030480 to R.R.R.).;supported in part by research grants from the 2019 Science and Technology Research Plan Project of the Chongqing Education Commission(China)(No.KJQN201900410);the 2019 Funding for Postdoctoral Research(Chongqing Human Resources and Social Security Bureau No.298);the Natural Science Foundation of China(No.82102696);supported by the Medical Scientist Training Program of the National Institutes of Health(No.T32 GM007281).

摘　　要：While progenitor cell-based cardiomyocyte regeneration holds great promise of repairing an injured heart,primary cardiomyogenic progenitors(CPs)have a limited life span in culture,hampering the use of CPs for in vitro and in vivo studies.We previously isolated primary CPs from mouse E15.5 fetal heart,and reversibly immortalized them with SV40 large T antigen(SV40 LTA),resulting in immortalized CPs(iCPs),which maintain long-term proliferation and ex-press cardiomyogenic markers and retain differentiation potential under appropriate differentiation conditions.

关 键 词：PROGENITOR cardio potential 

分 类 号：R32[医药卫生—人体解剖和组织胚胎学]